| Drosophilaimmigrans果蝇随机扩增DNA多态性初步分析 |
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| 引用本文： | 陈燕茹 许秀华. Drosophilaimmigrans果蝇随机扩增DNA多态性初步分析[J]. 北京大学学报(自然科学版), 1997, 33(2): 215-221 |
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| 作者姓名： | 陈燕茹 许秀华 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 为了探讨不同地理种群的果蝇在ＤＮＡ水平上的遗传多样性，推测秦岭地区伊米果蝇的起源，以主要来自中国的Ｄｒｏｓｏｐｈｉｌａｉｍｎｍｉｇｒａｎｓ种内７个不同地理种群的果蝇为材料和Ｄｒｏｓｏｐｈｉｌａｖｉｒｉｌｉｓ为对照，选取２０衙 １０ｂｐ长的寡聚核苷酸随机引物，进行随机扩增多态ＤＮＡ分析。
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| 关 键 词： | 果蝇 地理种群 RAPD 聚类分析 DNA 遗传多样性 |
Random Amplified Polymorfic DNA Analysis of Drosophila immigrans Geographic Strains |
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| CHEN Yanru XU Xiuhua ZHAO Zhongming ZHANG Wenxia DAI Zhuohua. Random Amplified Polymorfic DNA Analysis of Drosophila immigrans Geographic Strains[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1997, 33(2): 215-221 |
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| Authors: | CHEN Yanru XU Xiuhua ZHAO Zhongming ZHANG Wenxia DAI Zhuohua |
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| Abstract: | Random Amplified Polymorphic DNA (RAPD) was used to reveal the variation among Seven Drosophila immigrans geographic strains. Based on the RAPD markers the genetic distance among seven geographic strains of D. immigrans were computed and a phylogenetic tree was constructed. We found that: 1) Among D. immigrans strains, MU was further away to other strains than the others for it is the only one from Japan. 2) Within Chinese geographic strains, HY, SP(from Qinling area) are collected at the nearest geographic area and height above sea level, so they have little genetic variation. Another two high elevation strains TS(from Taishan)and LZ(from Lan Zhou)went together with HY and SP. It seems that: Higher elevational D. immigrans strains have more genetic resemblance. This phenomena happened recently during D. immigrans' speciation. |
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| Keywords: | Dosophila geographic strain RAPD phylogenetic analysis |
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