| 用于固相放射免疫分析的一类聚合物NHS酯的合成与表征 |
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| 引用本文： | 胡子文,谢家理,王科太,冯立,郝艳静.用于固相放射免疫分析的一类聚合物NHS酯的合成与表征[J].四川大学学报(自然科学版),2003,40(2):310-314. |
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| 作者姓名： | 胡子文 谢家理 王科太 冯立 郝艳静 |
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| 作者单位： | 四川大学化学学院,成都,610064 |
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| 基金项目： | 国家自然科学基金 (2 98710 2 2 ) |
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| 摘 要： | 将NHS(N—羟基琥珀酰亚胺)酯功能基团引入高分子载体上，合成了几种聚合物的NHS酯．研究了合成单体NHS酯及聚合物NHS酯的合成条件，测试了NHS酯及聚合物NHS酯的基本特性．该聚合物NHS酯可在温和条件下与蛋白反应，使蛋白以共价键结合方式固定在聚合物上，将为固相放免分析提供一种新材料．
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| 关 键 词： | 固相放射免疫分析 聚合物NHS酯 合成 共价结合 N—羟基琥珀酰亚胺 医学检验 |
| 文章编号： | 0490-6756(2003)02-0310-05 |
Synthesis and Caracterization of Polymer-NHS esters Used on Solid Phase Radioimmunoassay |
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| HU Zi wen,XIE Jia li,WANG Ke tai,FENG Li,HAO Yan jing.Synthesis and Caracterization of Polymer-NHS esters Used on Solid Phase Radioimmunoassay[J].Journal of Sichuan University (Natural Science Edition),2003,40(2):310-314. |
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| Authors: | HU Zi wen XIE Jia li WANG Ke tai FENG Li HAO Yan jing |
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| Abstract: | N Hydroxysuccinimide functional group was introduced into polymer carrier to synthesize a couple of polymer NHS esters. Conditions to prepare the NHS esters of all monomers and polymer NHS esters were studied. And these products' basic properties were also tested. The authors found that this polymer NHS ester can connect with proteins through covalent bounds under mild conditions and proteins can be immobilized on to polymers through covalent bounds. This technical method will offer a new material for radioimmunoassay. |
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| Keywords: | solid phase radioimmunoassay polymer NHS esters preparation covalent conjugation |
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