假单胞菌BS-03代谢产物的组成、结构与抗菌活性

为拓展生物发酵法制取糖脂类化合物在生物医药等方面的应用,该文自筛选经复合诱变的假单胞菌(Pseudonomas sp.)BS-03,以甘油为碳源,利用薄层层析(TLC)法鉴定其发酵液组分,采用傅里叶红外光谱(FTIR),电喷雾质谱(ESI-MS)和核磁共振(NMR)法分析发酵液提取物成分,并推测其化学结构.结果表明:发酵液组分主要由中性脂、糖脂和脂肽组成.糖脂提取物主要由4种鼠李糖脂构成,分别为RhC_(10)、RhC_(10)C_(10)、Rh_2C_(10)和Rh_2C_(10)C_(10),同时还含有多种自由酸前体及少量脱水自由酸前体.鼠李糖脂的抗菌活性试验结果显示鼠李糖脂的抗菌带较宽,对不同时期的微生物作用效果不同,其中霉菌的抑制效果最强,其次是酵母菌,效果最差的是细菌.

Components, Structure and Antimicrobial Activity of Metabolite of Pseudonomas sp. BS-03

The purpose is to expand the application of glycolipid compounds prepared by fermentation in the fields of biological medicine. Components in ferment liquor produced by compound mutation Pseudonomas sp. BS-03 with glycol as carbon source are identified with thin layer chromatography (TLC), in which the components of the extract of the ferment liquor are analyzed with Fourier transform infrared spectroscopy (FTIR), electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR), and the chemical structures of the ferment liquor are conjectured. The result shows that components in ferment liquor are mainly natural lipid, glycolipid and lipopeptide. There are four main rhamnolipids, namely RhC _(10) , RhC _(10)C _(10) , Rh_2C _(10) and Rh _2C _(10) C _(10) , in glycolipid extract, which contain both several kinds of acid precursors and dehydrating acid precursors. The testing result of antimicrobial activity of rhamnolipid shows that the diameter of inhibitory zone is wider and the impact on different phases of microbe is different. The mucedine has the strongest inhibitory effect, followed by the yeast, and the worst is the bacteria.