| HPLC测定蒙药清感九味丸中乌头碱的含量 |
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| 引用本文： | 包明兰,王秀梅,达古拉. HPLC测定蒙药清感九味丸中乌头碱的含量[J]. 内蒙古民族大学学报(自然科学版), 2008, 23(3): 329-332 |
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| 作者姓名： | 包明兰 王秀梅 达古拉 |
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| 作者单位： | [1]内蒙古民族大学蒙医药学院,内蒙古通辽028043; [2]呼伦贝尔市中蒙医医院,内蒙古海拉尔021000 |
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| 摘 要： | 目的：建立蒙药清感九味丸的质量标准．方法：用高效液相色谱法对处方中草乌有效成分乌头碱进行定量分析，采用VP-0D6（150L×4．6）柱；流动相为甲醇一水-氯仿-三乙胺（70：30：1：0．2V／V）；流速为0．5mL·min^-1；检测波长为240hm．结果：乌头碱进样量在0．60～3．Opg范围内与峰面积线性关系良好（r=0．9997）．平均加样回收率为99．28％（CV％=0．99％）．结论：本试验方法既先进又简便，结果准确可靠，可作为该制剂中乌头碱的含量测定方法．
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| 关 键 词： | 清感九味丸 HPLC 乌头碱 含量测定 |
Determination of the Content of Aconitine in Mongolian Medicine Qingganjiuwei Pill by HPLC |
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| BAO Ming-lan,WANG Xiu-mei,Dagul. Determination of the Content of Aconitine in Mongolian Medicine Qingganjiuwei Pill by HPLC[J]. Journal of Inner Mongolia University for the Nationalities(Natural Sciences), 2008, 23(3): 329-332 |
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| Authors: | BAO Ming-lan WANG Xiu-mei Dagul |
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| Affiliation: | BAO Ming -lan, WANG Xiu - mei, Dagul(1. College of Mongolian Medicine and Pharmacy, Inner Mongolia University for Nadonalities,, Tongliao 028043,China2. Hulunbttr Hospital for Traditional Chinese Medicine and Mongoliam Medicine, Hailar 021000 ,China) |
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| Abstract: | Objective： To establish the quality standard for Mongolian medicine Qingganjiuwei pill. Methods： Determining the aconitine content in Acontum Kusnezoffii by HPLC on the chro- matographic column of VP- ODS （150L × 4.6 ） and with methanol - water- chloroform - tri- ethylamine（70：30：1：0.2 V/V）as a mobile phase and flow rate of 0.5mL-min^-1. The detective wavelength was 240nm. Results：Obvious linear relation was showed between the input mass of a- conitine ranging from 0.60-- 0.30μg and the peak area（r = 0. 999 7）. The average rate of recovery for added sample was 99.28 % （CV% = 0.99 % ）. Conclusion： This method is not only simple but also advanced and the detection result is reliable. So it can be taken as a determination method to determine the content of aconitine in this preparation. |
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| Keywords: | Qingganjiuwei pill HPLC Aconitine Determination |
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