| 植物组织培养中真菌污染防治方法研究 |
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| 引用本文： | 程逸宇,郑建秋,迟卉,王幼芳.植物组织培养中真菌污染防治方法研究[J].贵州科学,2006,24(3):40-43. |
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| 作者姓名： | 程逸宇 郑建秋 迟卉 王幼芳 |
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| 作者单位： | 华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062 |
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| 摘 要： | 通过对组培接种室空气中真菌的分离和鉴定,发现曲霉(Aspergillus),毛霉(Mucor),青霉(Penicillum),木霉(Trichoderma),根霉(Rhizopus)等常见菌为组培的主要污染真菌.抑菌环实验表明次氯酸钠与制霉菌素抑菌效果较好.50mg/L酮康唑 50mg/L制霉菌素 0.1%次氯酸钠组成的混合杀菌剂,抑菌效果好,对植物伤害小,是良好的防真菌污染添加剂.
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| 关 键 词： | 组织培养 真菌污染 杀菌剂 |
| 文章编号： | 1003-6563（2005）04-0040-04 |
| 收稿时间： | 2005-09-23 |
| 修稿时间： | 2005年9月23日 |
PREVENTION OF THE FUNGAL CONTAMINATION IN PLANT TISSUE CULTURE |
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| CHENG Yi-yu,ZHEN Jian-qiu,CHI Hui,WANG You-fang.PREVENTION OF THE FUNGAL CONTAMINATION IN PLANT TISSUE CULTURE[J].Guizhou Science,2006,24(3):40-43. |
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| Authors: | CHENG Yi-yu ZHEN Jian-qiu CHI Hui WANG You-fang |
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| Institution: | Life Science College of East China Normal University, Shanghai 200062, China |
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| Abstract: | By the separation and identification of the epiphyte in the air of the plant tissue culture lab,Aspergillus,Mucor,Penicillum,Trichoderma,Rhizopus were found to be the main infective fungi in plant tissue culture. The experiment of mycostatic loop had indicated that sodium hypochlorite and nystatin are good fungistats.The mixture of 50mg/L ketoconazole 50mg/L nystatin 0.1% sodium hypochlorite is the best additive to prevent the fungal contamination in plant tissue culture and has slight damage to plants. |
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| Keywords: | tissue culture fungal contamination fungistat |
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