| 水稻黑条矮缩病毒基因组第九组分cDNA的克隆及序列分析 |
| |
| 引用本文： | 白逢伟,曲志才,许嘉,叶鸣明,许云敏,沈大棱.水稻黑条矮缩病毒基因组第九组分cDNA的克隆及序列分析[J].复旦学报(自然科学版),2001,40(6):692-694. |
| |
| 作者姓名： | 白逢伟 曲志才 许嘉 叶鸣明 许云敏 沈大棱 |
| |
| 作者单位： | 复旦大学生命科学学院遗传学研究所, |
| |
| 基金项目： | 美国麦氏 (McKnight)基金资助项目 |
| |
| 摘 要： | 从我国发病的玉米材料中提取水稻黑条矮缩病毒，抽提病毒RNA，利用RT-PCR等手段，获得了病毒基因组第九组分（S9）cDNA克隆。序列分析结果表明：S9全长1900bp，含有2个不重叠的ORF，编码蛋白的分子质量分别为39.9,24.2ku，与日本株S9核苷酸序列同源性为89%，与意大利株MRDV S8同源性为86%。
|
| 关 键 词： | 水稻黑条矮缩病毒 S9 cDNA 克隆 序列分析 玉米 编码蛋白 同源性 |
Screening of Peptides Binding to RSV S Protein by Phage Display |
| |
| BAI Feng-wei,?QU Zhi-cai?XU Jia,?YE Ming-ming,?XU Yun-min,?SHEN Da-leng.Screening of Peptides Binding to RSV S Protein by Phage Display[J].Journal of Fudan University(Natural Science),2001,40(6):692-694. |
| |
| Authors: | BAI Feng-wei ?QU Zhi-cai?XU Jia ?YE Ming-ming ?XU Yun-min ?SHEN Da-leng |
| |
| Abstract: | Rice black streaked dwarf virus (RBSDV) and its RNA were extracted from infected maize plants grown in China. Virus genome segment 9 (S9) cDNA clone was obtained by RT-PCR.Sequence analysis results showed that RBSDV S9 (1 900 bp) contained two non-overlapping ORFs encoding 39.9 ku and 24.2 ku proteins respectively. The sequence homology was 89% between Chinese RBSDV S9 and Japanese RBSDV S9 and 86% between Chinese RBSDV S9 and Italian MRDV S8. |
| |
| Keywords: | RBSDV S9 cDNA cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
