| 组培条件对油樟试管苗玻璃化的影响 |
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| 引用本文： | 魏琴,周黎军,宣朴,岳春芳,周锦霞,张新申. 组培条件对油樟试管苗玻璃化的影响[J]. 四川师范大学学报(自然科学版), 2006, 29(5): 606-608 |
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| 作者姓名： | 魏琴 周黎军 宣朴 岳春芳 周锦霞 张新申 |
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| 作者单位： | 四川大学,轻纺与食品学院,四川,成都,610065;宜宾学院,生物工程系,四川,宜宾,644007;宜宾学院,生物工程系,四川,宜宾,644007;四川省农科院,生物技术与核技术研究所,四川,成都,610066;四川大学,轻纺与食品学院,四川,成都,610065 |
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| 基金项目： | 四川省应用基础研究计划;四川省教育厅资助项目 |
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| 摘 要： | 研究了外植体年龄、处理方式对油樟试管苗玻璃化的影响及封口膜的透气性和培养基中不同浓度的蔗糖、琼脂、细胞分裂素（BA）浓度等因素对油樟试管苗玻璃化的影响．结果表明，以当年生带芽茎段为外植体玻璃化苗少，污染少；玻璃化苗的数量随着糖分及琼脂浓度的降低、BA浓度的增加而加重；封口膜的透气性与油樟苗的玻璃化关系不大．通过在培养基中适当调整蔗糖、琼脂、BA的浓度，可有效地控制油樟玻璃化苗数．
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| 关 键 词： | 油樟 玻璃化现象 组织培养 |
| 文章编号： | 1001-8395（2006）05-0606-03 |
| 收稿时间： | 2005-09-18 |
| 修稿时间： | 2005-09-18 |
Study on the Vitrification of Cinnamomum longepaniculatum (Gamble) N. Chao in Tissue Culture |
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| WEI Qin,ZHOU Li-jun,XUAN Pu,YUE Chun-fang,ZHOU Jin-xia,ZHANG Xin-shen. Study on the Vitrification of Cinnamomum longepaniculatum (Gamble) N. Chao in Tissue Culture[J]. Journal of Sichuan Normal University(Natural Science), 2006, 29(5): 606-608 |
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| Authors: | WEI Qin ZHOU Li-jun XUAN Pu YUE Chun-fang ZHOU Jin-xia ZHANG Xin-shen |
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| Affiliation: | 1. College of Light Industry and Food Engineering Sciences, Sichuan University, Chengdu 610065, Sichuan; 2. Department of Biological Engineering, Yibin University, Yinbin 644007, Sichuan ; 3. Institute of Biotechnology and Nuclear Technique, Academy of Agricultural Science, Chengdu 610066, Sichuan |
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| Abstract: | The effects of explant age and treatment,mixing sugar concentration,agar concentration,BA concentration in culture medium and gas permeability on vitrification were investigated.When current growth stems with buds were used as explants,the numbers of vitrified or polluted shoots were less than that of the others.The numbers of vitrified plantlet increased with decreasing of sugar and agar concentration and increasing of BA concentration.The gas permeability of seal membrane almost has no relation to vitrification.The numbers of vitrified plantlet of Cinnamomum longepaniculatum(Gamble)N.Chao could be controlled by manipulating the concentrations of sugar,agar and BA in the culture medium. |
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| Keywords: | Cinnamomum longepaniculatum(Gamble) N.Chao Vitrification Tissue culture |
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