| 链格孢菌粗毒素的提取方法和稳定性的研究 |
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| 引用本文： | 万佐玺. 链格孢菌粗毒素的提取方法和稳定性的研究[J]. 湖北民族学院学报(自然科学版), 2002, 20(4): 26-28 |
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| 作者姓名： | 万佐玺 |
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| 作者单位： | 湖北民族学院生物科学与技术学院 湖北恩施445000 |
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| 基金项目： | 农业部九五高技术和基础研究项目 ( 96 0 2 0 5 )，国家教委博士点基金项目 ( 0 2 -0 3-0 31) . |
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| 摘 要： | 以石油醚、乙酸乙酯、氯仿三种极性不同的溶济对501菌株的菌丝和培养液进行萃取，获取粗毒素，结果表明，501菌株产生的毒素是分泌培养液中的，是胞外毒素，且乙酸乙酯的萃取效果最好，回收率可达92．7％，萃取溶剂pH值对萃取结果没有影响。通过分析贮存时间、温度、pH对粗毒素稳定性的影响，表明贮存时间、温度、pH对毒素稳定性影响很小，有一定的开发价值。
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| 关 键 词： | 键格孢菌 毒素 提到 稳定性 |
| 文章编号： | 1008-8423(2002)04-0026-03 |
| 修稿时间： | 2002-08-25 |
Study on the Extracting Method and Stability of Alternaria Alternata Toxin |
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| WAN Zuo-xi. Study on the Extracting Method and Stability of Alternaria Alternata Toxin[J]. Journal of Hubei Institute for Nationalities(Natural Sciences), 2002, 20(4): 26-28 |
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| Authors: | WAN Zuo-xi |
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| Abstract: | The crude toxin was extracted from hypha and culture solution of Isolate 501 through three different polarity solvent: benzinum puncificatum, ethyl acetate and chloroform. The result showed that the toxin secreted by Isolate 501 existed in culture solution and the exacting effect of ethyl acetate with 92.7 percentage of recovery was the best. pH of exacting solvent had no effect on extraction .Analysis of storage time, temperature, pH indicated that they had little impact on the stability of toxin and this means that this kind of toxin has certain development value. |
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| Keywords: | Alternaria alternata toxin extraction stability |
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