胡桃醌含药血清体外抗肿瘤实验研究

研究胡桃醌(Juglone)含药血清对人肺癌A549细胞的生长抑制作用及对细胞周期的影响. 以血清药理学原理为基础,通过MTT法检测胡桃醌对A549细胞的生长抑制作用,并筛选最佳药物浓度;利用流式细胞仪分析胡桃醌对A549细胞周期的影响,并用Western blot验证. 实验结果表明,胡桃醌的半衰期T_1/2=136 min,MTT法显示其对体外培养的人肺癌A549细胞具有明显抑制作用,比较12,24,48 h细胞存活率得出10%高剂量组(0.107 μg/mL)胡桃醌血清抑制作用较好,同时,胡桃醌可通过凋亡途径阻滞细胞于S期,增加caspase-3的表达. 胡桃醌对A549细胞可能具有生长抑制作用,将细胞阻滞在S期,诱导细胞凋亡,上调caspase-3表达量. 

Experimental Study of Serum Containing Juglone Anti-Tumor in Vitro

The effects of serum containing Juglone on inhibiting the growth of A549 cells and cell cycle in vitro were experimentally researched. On the basis of seropharmacology, MTT assay was used to detect the growth inhibition of A549 cells by the interference of serum containing Juglone and to screen the best drug concentration. The effect of Juglone on A549 cell cycle was analyzed by FCM (flow cytomery) apparatus and then was verified by Western blot experiment. Experiment shows that Juglone's half-time is 136 min. Result of MTT assay indicates, by comparing 12, 24 and 48 h cell survival rates, the group with 10% high dose of serum containing Juglone has the best efficacy. Meanwhile, Juglone could induce apoptosis of A549 cells, arrest cells in S phase and improve the expression of Caspase-3. It is concluded from experiments that Juglone could significantly inhibit the growth of A549 cells, arrest cells in S phase, induce apoptosis and improve the expression of Caspase-3.