| 大豆根瘤内感染细胞原生质体的分离与活力检测 |
| |
| 引用本文： | 李有忠,强科斌.大豆根瘤内感染细胞原生质体的分离与活力检测[J].青海师范大学学报(自然科学版),1994(2):51-54. |
| |
| 作者姓名： | 李有忠 强科斌 |
| |
| 作者单位： | 青海师范大学，甘肃农业大学 |
| |
| 摘 要： | 作者在前人工作的基础上，分离了大豆根瘤中感染细胞的原生质体，分离得到的感染细胞原生质体产量较高。经显微观察和几种细胞化学方法检测的结果表明，原生质体纯度高、破损少、具很强的生活力。对于以其为材料的生化研究具有一定的实际应用价值。
|
| 关 键 词： | 大豆 根瘤 原生质体 分离 感染细胞 活力 |
ISOLATION OF INFECTED CELL PROTOPLASTS FROM SOYBEAN ROOT NODULES |
| |
| In Youzhong,Zhang De,Qiang Kebin.ISOLATION OF INFECTED CELL PROTOPLASTS FROM SOYBEAN ROOT NODULES[J].Journal of Qinghai Normal University(Natural Science Edition),1994(2):51-54. |
| |
| Authors: | In Youzhong Zhang De Qiang Kebin |
| |
| Institution: | In Youzhong;Zhang De;Qiang Kebin(Qinghai Normal University)(Gansu Agriculture University) |
| |
| Abstract: | e presented here a modified method for isolating infected cell protoplasts from Soybean (Glycine or(L.) Merr cv.Bragg) root nodules. The protoplasts isolated with the method were assayed by using microscope with or without Fluorescein diacetate (FDA) and Evans Blue staining. The results showed that infected cells were very large with a high density,irregular in shape and obviously pink in color,that protoplast preparation was highly pure with little cross contamination from uninfected cells and that integrity and the viability of isolated protoplsts were very high as well. The method could be practical and useful in studies in which the protopalsts would be employed. |
| |
| Keywords: | Glycine max root nodule protoplasts isolation |
| 本文献已被 CNKI 维普 等数据库收录！ |
