| 喂食Cry1Ac毒素对棉铃虫中肠类胰蛋白酶的影响 |
| |
| 引用本文： | 袁灿,夏立秋,丁学知,田野,尹佳.喂食Cry1Ac毒素对棉铃虫中肠类胰蛋白酶的影响[J].湖南师范大学自然科学学报,2009,32(2). |
| |
| 作者姓名： | 袁灿 夏立秋 丁学知 田野 尹佳 |
| |
| 作者单位： | 湖南师范大学生命科学学院,微生物分子生物学湖南省重点实验室,中国,长沙,410081 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，教育部高等学校博士学科点专项科研基金，湖南省自然科学基金优秀面上资助项目 |
| |
| 摘 要： | 苏云金芽胞杆菌(Bacillus thuringiensis,Bt)在芽胞形成过程中产生的原毒素被敏感昆虫吞食进入消化道后,在中肠蛋白酶的作用下经历一系列酶的剪切活化过程,形成具有杀虫活性的毒性肽.类胰蛋白酶因其在昆虫中肠内蛋白质水解和Bt毒素活化中起着重要作用而受到广泛研究.本研究提取了棉铃虫4龄幼虫的中肠BBMV蛋白,利用SDS-PAGE分析鉴定到喂食Cry1Ac毒素24 h后,棉铃虫幼虫中肠类胰蛋白酶的表达量呈明显下降趋势.该下降趋势很有可能是作为一种昆虫对Bt毒素作用的早期反应,通过影响Bt毒素在中肠中的酶解活化过程,从而为昆虫提供即时保护.
|
| 关 键 词： | 苏云金芽胞杆菌 棉铃虫 类胰蛋白酶 |
Influence of the Ingestion of Cry1Ac Toxin on Trypsin-Like Protease in Helicoverpa Armigera Midgut |
| |
| YUAN Can,XIA Li-qiu,DING Xue-zhi,TIAN Ye,YIN Jia.Influence of the Ingestion of Cry1Ac Toxin on Trypsin-Like Protease in Helicoverpa Armigera Midgut[J].Journal of Natural Science of Hunan Normal University,2009,32(2). |
| |
| Authors: | YUAN Can XIA Li-qiu DING Xue-zhi TIAN Ye YIN Jia |
| |
| Institution: | Key Laboratory of Microbial Molecular Biology of Hunan Province;College of Life Science;Hunan Normal University;Changsha 410081;China |
| |
| Abstract: | The protoxin produced by Bacillus thuringiensis (Bt) during the sporulation phase is cleaved by midgut proteases to a stable toxin core. Trypsin-like protease is widely studied because of its important role in the activation process. In this study, BBMV proteins from the fourth instar larvae of Helicoverpa armigera before and 24 h after ingestion of Bt Cry1Ac toxin are prepared. SDS-PAGE separation of the BBMV proteins shows a dramatic decrease in the level of trypsin-like protease 24 h after ingestion of B... |
| |
| Keywords: | Cry1Ac |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
