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Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR |
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| Authors: | Consortium for Influenza Study at Shanghai Shanghai-MOST Key Laboratory for Disease Heal |
| Institution: | YAN An1;DING GuoHui2;ZHOU ZhenFeng1;DONG Hui1;ZHANG YaKun1;ZHU Lei3;HE YunGang3;ZHANG GuoQing4;LI YiXue2;4;SUN Bing2;HUANG Zhong2;LAN Ke2;JIN Li1;2;5;WANG HongYan5;WANG XiaoNing5;6;YANG Zhong4;ZHONG Yang4;DAI JianXin7;8;GUO YaJun7;WANG Hao7;CHE XiaoYan9;WU Fan10;YUAN ZhenGan10;ZHANG Xi10;CAO ZhiWei4;11;ZHOU XiaoNong12;ZHOU JiaHai13;MA ZhiYong14;TONG GuangZhi14;ZHAO GuoPing1;3;JIN WeiRong3 & XIONG Hui1 |
| Abstract: | The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.T... |
| Keywords: | influenza A virus HA gene H1 and H3 subtypes specific amplification by RT-PCT DNA sequencing |
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