流动注射-化学发光分析法测定磺胺胍

为了实现快速测定样品中磺胺胍含量并降低检测成本,联合流动注射技术,建立了一种测定磺胺胍含量的新方法。基于在酸性条件下磺胺胍对高锰酸钾-甲醛体系的发光信号有明显的增强作用而建立了磺胺胍的检测体系并优化了各项参数。结果显示：在优化条件后,磺胺胍的线性范围为5×10^-5～6×10^-4 mol·L^-1;对浓度为2×10^-4 mol·L^-1的磺胺胍溶液进行11次平行测定,计算出检出限为6×10^-7 mol·L^-1,检测结果的相对标准偏差为1.1%;加标回收实验测得的回收率为97%～103%。该方法检测快速、灵敏度高且成本较低,可成功应用于样品中磺胺胍的测定。

Determination of Sulfaguanidine by Flow Injection Chemiluminescence Analysis

Although sulfadiguanide has a broad-spectrum antibacterial effect as a drug used in clinical practice, excessive intake is fatal to the human body, so in order to determine the content of sulfadiguanide in drugs and reduce the cost of detection, a new chemiluminescence method for the determination of sulfadiguanide content by combined flow injection technology was established. Under the acid condition, the sulfadiguanide is determined by potassium permanganate-formaldehyde luminescent system combined with flow injection chemiluminescence analyzer. The result show that the linear range is 5×10 -5 ～6×10 -4 mol·L -1 , the detection limit is 6×10 -7 mol·L -1 , the relative standard deviation is 1.1% ( n =11, C sulfadiguanide =2×10 -4 mol·L -1 ), the recovery is 97%～ 103%. Using this method has been successful to the detection of sulfadiguanide recognition with satisfactory result.