| 液体培养发状念珠蓝细菌代谢物提取方法的比较分析 |
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| 引用本文： | 韩培培,孙 莹,段文倩,石 拓,贾士儒.液体培养发状念珠蓝细菌代谢物提取方法的比较分析[J].天津科技大学学报,2014(1):7-10. |
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| 作者姓名： | 韩培培 孙 莹 段文倩 石 拓 贾士儒 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457 |
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| 基金项目： | 国家自然科学基金资助项目（31201405,31271809）;国家级“大学生创新创业训练计划”资助项目（201210057008） |
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| 摘 要： | 比较了基于气相色谱–质谱联用技术(GC-MS)发状念珠蓝细菌代谢物的2种不同提取方法.在对样品进行快速洗涤和淬灭后,分别采用冷甲醇法和甲醇/氯仿法对样品进行提取,并考察了样品量对提取效果的影响.结果显示,从提取到的代谢物种类和丰度角度,冷甲醇法的提取效果要优于甲醇/氯仿法,且采用50,mg样品量的提取效果较佳.通过本方法,在发状念珠蓝细菌中共检测到46种代谢物,包括19种有机酸、1种无机酸、13种氨基酸、4种胺类、4种醇类和5种糖类物质.
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| 关 键 词： | 发状念珠蓝细菌 蓝细菌 气相色谱–质谱联用技术 代谢物 提取方法 |
Comparison of Metabolites Extracting Methods from Liquid CulturedNostoc flagelliforme |
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| HAN Peipei,SUN Ying,DUAN Wenqian,SHI Tuo,JIA Shiru.Comparison of Metabolites Extracting Methods from Liquid CulturedNostoc flagelliforme[J].Journal of Tianjin University of Science & Technology,2014(1):7-10. |
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| Authors: | HAN Peipei SUN Ying DUAN Wenqian SHI Tuo JIA Shiru |
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| Institution: | HAN Peipei, SUN Ying, DUAN Wenqian, SHI Tuo, JIA Shiru |
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| Abstract: | With gas chromatography mass spectrometry（GC-MS）,two kinds of metabolites extracting methods ofNostoc flagelliforme were compared. After being washed and quenched,samples were extracted using the cold methanol method and methanol / chloroform method,respectively. The results suggested that the better extraction effect was achieved with the cold methanol method with the 50,mg sample. Through this method,46,kinds of metabolites from Nostoc flagelliformewerede-tected and identified,including 19,kinds of organic acids,1,kind of inorganic acid,13,kinds of amino acids,4,kinds of amines,4,kinds of alcohols,and 5,kinds of sugar. |
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| Keywords: | Nostoc flagelliforme cyanobacteria metabolite extracting methods |
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