| 用ELISA双抗体夹心法检测人重组白细胞介素2(rHuIL-2)在酵母菌中表达的研究 |
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| 引用本文： | 刘启鼎,顾海东,项文凯. 用ELISA双抗体夹心法检测人重组白细胞介素2(rHuIL-2)在酵母菌中表达的研究[J]. 复旦学报(自然科学版), 1993, 0(3) |
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| 作者姓名： | 刘启鼎 顾海东 项文凯 |
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| 作者单位： | 复旦大学微生物学系(刘启鼎，顾海东)，复旦大学微生物学系(项文凯) |
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| 摘 要： | 采用ELISA夹心法检测人重组IL-2在酵母菌中表达产物的免疫学活性.结果表明,本法具有特异性强、简易、快速和重复性好等特点,尤其对利用工程菌制备及纯化rlL-2过程中能较快地监测出是否有该产物表达.
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| 关 键 词： | 重组白细胞介素2 ELISA夹心法 单克隆抗体 |
STUDY ON DETECTION OF RECOMBINANT HUMAN INTERLEUKIN-2(rHuIL-2)IN YEAST BY SANDWICH ELISA |
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| Liu Qiding,Gu Haidong,Xiong Wenkai. STUDY ON DETECTION OF RECOMBINANT HUMAN INTERLEUKIN-2(rHuIL-2)IN YEAST BY SANDWICH ELISA[J]. Journal of Fudan University(Natural Science), 1993, 0(3) |
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| Authors: | Liu Qiding Gu Haidong Xiong Wenkai |
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| Affiliation: | Department of Microbiology |
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| Abstract: | A Sandwich ELISA Method for measurement of the activity of recombinant human IL-2 expressed in yeast was established. The results showed that it was highly specific,simple,rapid,sensitive and reproducible.The method could be used in the constructional engineering bacteria preparation and purification of rIL-2. |
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| Keywords: | recombinant interleukin-2 sandwich ELISA monoclonal antibody |
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