| 在线富集毛细管区带电泳测定唾液中的N-乙酰神经氨酸 |
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| 引用本文： | 贾丽,周维,高文华. 在线富集毛细管区带电泳测定唾液中的N-乙酰神经氨酸[J]. 汕头大学学报(自然科学版), 2004, 19(3): 42-47 |
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| 作者姓名： | 贾丽 周维 高文华 |
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| 作者单位： | 汕头大学理学院化学系中心实验室,汕头,515063 |
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| 基金项目： | 汕头大学校基金资助项目 (NO :1 3 0 5 1 2 0 40 ) |
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| 摘 要： | 建立了一种测定N 乙酰神经氨酸的在线富集毛细管区带电泳法 .该方法用 40mmol/L磷酸二氢钠作为缓冲溶液电解质 ,通过将样品溶解在乙腈∶水 ( 2∶1 )的样品基体中 ,进样时间增加到 1 0 0s来达到样品富集的效果 ,并考察了样品基体中乙腈浓度和进样时间对富集效果的影响 .研究了缓冲溶液pH值、三种不同电解质和有机修饰剂的加入对N 乙酰神经氨酸检测的影响 .最后考察了所建立方法的线性、检测限和重现性 ,并将该方法成功地应用于测定人体唾液中的N 乙酰神经氨酸 ,在 1 0min内完成分离 .
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| 关 键 词： | 毛细管区带电泳法 在线富集 N-乙酰神经氨酸 唾液 |
| 文章编号： | 1001-4217(2004)03-0042-06 |
| 修稿时间： | 2004-01-05 |
Online Concentration and Determination of N-Acetyneuraminic Acid in Saliva Using Capillary Zone Electrophoresis |
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| JIA Li,ZHOU Wei,GAO Wen-hua. Online Concentration and Determination of N-Acetyneuraminic Acid in Saliva Using Capillary Zone Electrophoresis[J]. Journal of Shantou University(Natural Science Edition), 2004, 19(3): 42-47 |
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| Authors: | JIA Li ZHOU Wei GAO Wen-hua |
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| Abstract: | An on-line concentration capillary zone electrophoresis method was developed to determine N-acetyneuraminic acid in saliva.The influences of three different electrolytes,buffer pH and organic modifiers on the determination of N-acetyneuraminic acid were studied.The effects of the composition of sample matrix and injection time on the concentration efficiency of N-acetyneuraminic acid were also studied.Calibration line,detection limit and reproducibility of the developed method were investigated and the method was applied to determine N-acetyneuraminic acid in saliva.The separation was finished within 10*!min. |
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| Keywords: | capillary zone electrophoresis online concentration N-acetyneuraminic acid saliva |
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