蛇足石杉芽胞萌发及生长发育的影响因素

为研讨蛇足石杉芽胞萌发及生长发育的影响因素，试验分析了培养温度、培养基和光照强度以及赤霉素对蛇足石杉芽胞萌发及生长发育的影响.试验结果表明，蛇足石杉芽胞萌发最佳培养温度为(22±2)℃；最佳培养基为Moore，萌发率约82%，植株平均高度约14.620 mm；Knudson C培养基次之，萌发率约64%，植株平均高度约为5.158 mm；暗培养适宜芽胞萌发及不定根生长，萌发率高达92%，平均根长9.088mm，800 lx弱光照培养不定根生长缓慢，平均根长8.675 mm，1 600 lx光照对不定根的生长有一定的抑制作用；赤霉素对于芽胞萌发与生长有一定抑制作用.建立了蛇足石杉芽胞萌发培养体系，为进一步研究蛇足石杉生长发育、生理生化变化提供参考.

Factors of Influencing Gemmae Germination and Growth of Huperzia Serrata

The factors of influencing the gemmae germination of Huperzia serrata and growth condition are explored.The effect of culture medium,culture temperature,and light intensity and GA₃ on the gemmae germination condition of Huperzia was studied,and the growth morphogenesis during the course of gemmae germination of Huperzia serrata was observed at the same time.Results showed that the best culture temperature for the gemmae germination was (22±2)℃,and the germination rate of the optimum medium for Moore,the germination rate was as high as 82%,and the average plant length could be up to 14.620 mm.The second culture medium for the gemmae germination was Knudson C,the germination rate was 64%,and the average adventitious root length was about 5.158 mm.Darkness was conducive to gemmae germination,the germination rate was 92%,and the average adventitious root length was about 9.088 mm.Under 800lx weak light,the adventitious root grew slowly,and the adventitious root length could be up to 8.675 mm.1600lx light irradiation exhibited some inhibition for the adventitious root growth.And GA₃ for gemmae germination and growth exhibited some inhibition. This study establishes the gemmae germination culture system for Huperzia serrata,which consitutes the foundation for further study on the growth,physiological and biochemical change of Huperzia serrata.