一株高产丁二酮菌株的筛选与鉴定

以湖南省湘西地区土家腊肉中分离的41株细菌为出发菌，采用平板显色法和邻苯二胺比色法筛选高产丁二酮的微生物菌种.通过菌落形态观察、生理生化特征测定和基于16S rRNA基因序列分析，对分离菌株进行鉴定.结果表明，7株细菌能产生较高含量的丁二酮，其中菌株BCL-8产丁二酮量最高，在葡萄糖蛋白胨水培养基中发酵20 h，丁二酮质量浓度达68.9 mg/L，该菌经鉴定为地衣芽孢杆菌（Bacillus licheniformis）.菌株BCL-8是一株优良的产丁二酮的微生物种质资源.

Screening and Identification of High Butanedione-Yielding Bacterial Strain

To obtain the high butanedione-yielding bacterial strain,41 strains of bacteria isolated from traditional Tujia bacon were screened by way of V-P color reaction and 1，2-diaminobenzene colorimetric method.The isolated strains were identified by bacterial morphological,physiological and biochemical characteristics，and phylogenetic relationships based on 16S rRNA gene sequences.It was found that 7 strains of bacteria could produce higher content of butanedione，of which strain BCL-8 had the highest yield of butanedione.When strain BCL-8 had been fermented in glucose and peptone medium for 20 hours，butanedione content in fermentation liquor was determined to be 68.9 mg/L.Strain BCL-8 was identified as Bacillus licheniformis based on its morphological，physiological and biochemical characteristics，and 16S rRNA gene sequences analysis.The results showed that strain BCL-8 is a superior butanedione-producing microorganism.