介孔羟基磷灰石纳米粒子的制备及作为蛋白类缓释药物载体的应用

利用气液沉淀和水热法相结合制备介孔羟基磷灰石(HA)纳米粒子,并采用X射线衍射(XRD)、透射电子显微镜(TEM)、比表面分析(BET)、孔径计算(BJH)、傅里叶红外(FT-IR)对其进行表征.研究牛血清白蛋白在羟基磷灰石上的等温吸附,通过对等温吸附曲线的拟合,发现该吸附符合Langmuir模型.同时测定了羟基磷灰石-牛血清白蛋白复合物在不同pH磷酸缓冲液下的体外释放行为,结果表明不同pH下释放率的不同是电荷相斥效应造成的,且体外释放行为符合Korsmeyer-Peppas释放动力学方程,其释放机制符合非Fickian扩散.

Preparation of mesoporous hydroxyapatite nanoparticles and application as a controllable release carrier of protein

Mesoporous hydroxyapatite (HA ) nanoparticles were synthesized via gas‐liquid chemical precipitation combining with hydrothermal method .And the structure ,morphology and pore size distribution of HA were observed and measured using the X‐ray powder diffraction (XRD ) , transmission electron microscopy (TEM ) , Brunauer‐Emmett‐Teller ( BET ) , Barret‐Joyner‐Halenda scheme ( BJH ) and Fourier transform infrared spectrophotometer (FT‐IR) .The adsorption isotherms of bovine serum albumin (BSA) on as‐prepared HA at various temperatures were obtained and the corresponding equilibrium data fitted better the Langmuir model better .Meanwhile ,the BSA cumulative release rate of the BSA‐loaded HA was investigated .The release rate was different at different pH values due to the effect of charge repulsion .Furthermore ,the vitro release was in accordance with the Korsmeyer‐Peppas equation and the release mechanism fitted the non‐Fickian diffusion .