| 2个棒状杆菌质粒pXZ10142和pXZ10145.1及其转座子TN45的特征分析 |
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| 引用本文： | 任兆瑞,施少林,蒋晓兵,严维耀,雷呈祥,刘明秋,郑兆鑫.2个棒状杆菌质粒pXZ10142和pXZ10145.1及其转座子TN45的特征分析[J].复旦学报(自然科学版),2004,43(2):200-205. |
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| 作者姓名： | 任兆瑞 施少林 蒋晓兵 严维耀 雷呈祥 刘明秋 郑兆鑫 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,遗传工程国家重点实验室,上海,200433;海军医学研究所,上海,200433 |
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| 摘 要： | 对1株棒状杆菌1014中分离到的2个质粒pXZ10145．1和pXZ10142进行了相互关系分析．序列分析显示pXZ10142源自pXZ10145．1．pXZ10145．1包括6个开放读码框(open reading frame，ORF)，其中ORF1编码247个氨基酸，经缺失分析是复制酶．在pXZ10145．1上发现1个转座子TN45，转座子上有CMR基因和TNP基因．一对颠倒重复序列IR1a和IR1b位于pXZ10145．1和pXZ10142的交互处，两边是顺向重复序列ATCTAG．
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| 关 键 词： | 棒状杆菌 质粒 pXZ10145.1 pXZ10142 转座子Tn45 |
| 文章编号： | 0427-7104(2004)02-0200-06 |
Complete Nucleotide Sequence Analysis of Two Corynebacterial Plasmids pXZ10142 and pXZ10145 Containing a Transposon-like Sequence Tn45 |
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| REN Zhao-rui.Complete Nucleotide Sequence Analysis of Two Corynebacterial Plasmids pXZ10142 and pXZ10145 Containing a Transposon-like Sequence Tn45[J].Journal of Fudan University(Natural Science),2004,43(2):200-205. |
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| Authors: | REN Zhao-rui |
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| Institution: | REN Zhao-rui~ |
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| Abstract: | Two plasmids, pXZ10145.1 and pXZ10142, isolated from one strain of Corynebacterium glutamicum 1014 were analysed. Comparison between the pXZ10145.1 and pXZ10142 sequences showed that the plasmid pXZ10142 originated in the excision from the plasmid pXZ10145.1. The sequence of pXZ10145.1 revealed 6 open reading frames (ORFs). ORF1 encoding 247 amino acids encompassed the replication protein, which was proved by deletion analysis. A transposon-like structure Tn45 was implied from the DNA sequence of pXZ10145.1. It contained a CMR gene, a TNP gene, and two inverted repeat sequences IR1a and IR1b, which are located at the junction between (pXZ10145.1) and pXZ10142. The direct repeat sequences are ATCTAG flanking both inverted repeat sequences of IR1a and IR1b. |
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| Keywords: | Corynebacterium glutamicum plasmid pXZ10145 1 pXZ10142 transposon Tn45 |
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