| 细茎大豆（G.gracilis）rbcS基因结构与分子进化分析 |
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| 引用本文： | 张二荃 王喜萍. 细茎大豆（G.gracilis）rbcS基因结构与分子进化分析[J]. 复旦学报(自然科学版), 1998, 37(2): 151-156 |
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| 作者姓名： | 张二荃 王喜萍 |
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| 作者单位： | 复旦大学(张二荃，王喜萍，曹凯鸣，顾其敏)，吉林省农科院大豆所(庄炳昌) |
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| 摘 要： | 从细茎大豆的嫩叶中提取总ＤＮＡ，用ＰＣＲ方法扩增得到包含完整编码区的ｒｂｃＳ基因并将其克隆到ｐＢＬＵＥＳＣＲＩＰＴ载体中。完成全基因１０８９个核苷酸测序后，运用ＰＣＧＥＮＥ进行顺序编辑和同源比较，并应用ＭＥＧＡ１．０２１软件中的Ｎｅｉｇｈｂｏｒ－ｊｏｉｎｉｎｇ方面画出Ｒｕｂｉｓｃｏ小亚基仓的系统进化树。
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| 关 键 词： | 大豆 序列分析 分子进化 rbcs基因 细茎大豆 |
The Structure and Evolution Analysis of G. gracilis rbcS Gene |
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| Zhang Erquan, Wang Xiping, Cao Kaiming, Gu Qimin, Zhuang Bing Chang. The Structure and Evolution Analysis of G. gracilis rbcS Gene[J]. Journal of Fudan University(Natural Science), 1998, 37(2): 151-156 |
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| Authors: | Zhang Erquan Wang Xiping Cao Kaiming Gu Qimin Zhuang Bing Chang |
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| Abstract: | Total DNA was extracted from tender leaves of semi-cultural soybean (G. gracilis). The rbcS containing the whole coding region for Rubisco small subunit was amplified by PCR and then cloned and sequenced. This gene was subjected to homology analysis from PCGENE package, and the phylogenetic tree of Rubisco small subunit from different plants was constructed by neighbor-joining method of MEGA 1. 02 package. |
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| Keywords: | soybean rbcS sequence analysis neighbor-joining method molecular evolution |
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