| HPLC法测定鞣酸苦参碱中3种生物碱的含量 |
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| 引用本文： | 麻秀萍,蒋朝晖,贾宪生,张明昶,徐文芬,袁瑶. HPLC法测定鞣酸苦参碱中3种生物碱的含量[J]. 贵州科学, 2012, 30(5): 23-26 |
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| 作者姓名： | 麻秀萍 蒋朝晖 贾宪生 张明昶 徐文芬 袁瑶 |
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| 作者单位： | 贵阳中医学院,贵阳,550002 |
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| 基金项目： | 2003年贵州省中药现代化项目:安肠止泻胶囊工艺和质量标准提高研究 |
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| 摘 要： | 为控制鞣酸苦参碱的质量,建立高效液相色谱法(HPLC)测定鞣酸苦参碱中生物碱的含量。方法:采用HPLC法测定鞣酸苦参碱中3个主要生物碱的含量。结果:苦参碱进样量线性范围0.03132～0.1488μg,平均回收率为97.28%,RSD为0.88%;氧化槐果碱线性范围0.01938～0.2132μg,平均回收率为101.1%,RSD为1.03%;氧化苦参碱线性范围0.1751～1.926μg,平均回收率为99.51%,RSD为0.71%。结论:该法专属性强,准确稳定,重现性好,可作为本品的质量控制方法。
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| 关 键 词： | 鞣酸苦参碱 高效液相色谱法 苦参碱 氧化槐果碱 氧化苦参碱 |
Determination of 3-Kind Alkaloid Content in Tannin Matrine by HPLC |
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| MA Xiu-ping,JIANG Zhao-hui,JIA Xian-sheng,ZHANG Ming-chang,XU Wen-fen,YUAN Yao. Determination of 3-Kind Alkaloid Content in Tannin Matrine by HPLC[J]. Guizhou Science, 2012, 30(5): 23-26 |
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| Authors: | MA Xiu-ping JIANG Zhao-hui JIA Xian-sheng ZHANG Ming-chang XU Wen-fen YUAN Yao |
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| Affiliation: | ( Guiyang College of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China) |
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| Abstract: | A high performance liquid chromatography （HPLC） was adopted to determine tannin matrine in alka- loid content to control the quality of tannin matrine. Method ： HPLC was used for determination of tannin matrine in three main alkaloid contents. Results： The standard curve for matrine was linear in the range of 0. 03132 - 0. 1488 p,g, the average recovery was 97.28 % and RSD was 0.88 %. The standard curve for oxysophocarpine was linear in the range of 0. 01938 - 0. 2132 μg, the average recovery was 101.1% and RSD was 1.03 %. The standard curve for oxymatrine was linear in the range of 0. 1751 - 1. 926 μg, the average recovery was 99.51% and RSD was 0.71%. Conclusion： Being specific, accurate and stable, the method could be used as quality con- trol for tannin matrine. |
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| Keywords: | tannin matrine high performance liquid chromatography matrine oxysophocarpine oxymatrine |
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