| 利用有机溶剂法快速纯化绿色荧光蛋白 |
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| 引用本文： | 王盛,董洁,曾瑾,曹悠,张虹,雒丽娜. 利用有机溶剂法快速纯化绿色荧光蛋白[J]. 宁夏大学学报(自然科学版), 2012, 0(4): 400-404 |
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| 作者姓名： | 王盛 董洁 曾瑾 曹悠 张虹 雒丽娜 |
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| 作者单位： | [1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021 |
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| 基金项目： | 国家自然科学基金资助项目（31060023）;宁夏回族自治区大学生创新基金资助项目 |
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| 摘 要： | 介绍非色谱GFP纯化方法——有机溶剂纯化法．该方法在短时间内（30min）即可获得大量较高纯度（91．7％），且荧光光谱特征不改变的GFP蛋白．纯化过程简单，无需昂贵的仪器和设备，适合绝大多数中小实验室使用．
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| 关 键 词： | 绿色荧光蛋白 表达与纯化 有机提取 两相系统 |
Rapid Purification of Green Fluorescent Protein from Escherichia Coli by Organic Extraction |
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| Wang Sheng,Dong Jie,Zeng Jin,Cao Min,Zhang Hong,Luo Lina. Rapid Purification of Green Fluorescent Protein from Escherichia Coli by Organic Extraction[J]. Journal of Ningxia University(Natural Science Edition), 2012, 0(4): 400-404 |
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| Authors: | Wang Sheng Dong Jie Zeng Jin Cao Min Zhang Hong Luo Lina |
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| Affiliation: | 1. School of Life Science, Ning Xia University, Yinchuan 750021, China; 2. Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, Ning Xia University, Yinchuan 750021, China) |
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| Abstract: | Green fluorescent protein （GFP） is widely used as an excellent reporter molecule and cell biology. Some biochemical and immunological assays require high-purity GFP. in biochemistry However, the majority of current procedures for GFP purification includes multiple time-consuming chromatography steps with a low yield of the desired product or requires tag-containing proteins. In the present work, an alternative method is described for the GFP purification using organic extraction. The method is easily scalable for yielding a highly homogeneous GFP protein indistinguishable in spectroscopic properties from the origin one. |
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| Keywords: | green fluorescent protein expression and purification organic extraction~ two phase water/or ganic system |
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