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| 甘蔗蔗糖磷酸合成酶基因启动子遗传转化烟草的研究 | |
| 引用本文： | 高玉娜,林荣华,周平,叶冰莹,陈由强,陈如凯.甘蔗蔗糖磷酸合成酶基因启动子遗传转化烟草的研究[J].福建师范大学学报(自然科学版),2010,26(1). |
| 作者姓名： | 高玉娜 林荣华 周平 叶冰莹 陈由强 陈如凯 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108;发育与神经生物学福建省高等学校重点实验室,福建,福州,350108 2. 福建省农业科学院果树研究所,福建,福州,350013 3. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108 |
| 基金项目： | 国家自然科学基金资助项目，国家高技术研究发展计划"863"课题，福建省科技厅资助项目 |
| 摘 要： | 蔗糖磷酸合成酶(Sucrose Phosphate Synthase,SPS)是调节甘蔗蔗糖合成的关键酶之一.根据课题组已经构建的甘蔗SPS5侧翼序列驱动GUS表达的载体,通过根癌农杆菌介导的叶盘转化法,转化模式植物烟草,获得26棵抗性植株.抗性植株的PCR结果表明,获得3株转基因植株.GUS染色分析说明,SPS的5侧翼序列可以驱动GUS基因表达,SPS的5侧翼序列具有启动子活性. |
| 关 键 词： | 蔗糖磷酸合成酶基因Ⅲ 启动子 转基因 sucrose phosphate synthase Ⅲ |
Research of Sugarcane Sucrose Phosphate Synthase Gene Promoter Using Genetic Transformation in Tobacco |
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| GAO Yu-na,LIN Rong-hua,ZHOU Ping,YE Bing-ying,CHEN You-qiang,CHEN Ru-kai.Research of Sugarcane Sucrose Phosphate Synthase Gene Promoter Using Genetic Transformation in Tobacco[J].Journal of Fujian Teachers University(Natural Science),2010,26(1). | |
| Authors: | GAO Yu-na LIN Rong-hua ZHOU Ping YE Bing-ying CHEN You-qiang CHEN Ru-kai |
| Abstract: | Sucrose phosphate synthase is one of the key enzymes controling biosynthesis of sucrose in Sugarcane. According to the expression vector flanking sequence of SPSⅢ of sugarcane, 26 resistant tobaccoes were obtained by Agrobacterium-mediated leaf disc transformation method. The result of PCR from resistant tobaccoes leaves indicated that 3 transgenic plants were obtained. The analysis of GUS staining shows that the in transgenic tobaccoes,it has promoter activity. |
| Keywords: | GUS promoter GUS transgenic |
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