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MT—hGH基因注入小鼠受精卵的研究
引用本文:扈廷茂,刘明秋,等.MT—hGH基因注入小鼠受精卵的研究[J].内蒙古大学学报(自然科学版),1993,24(1):75-80.
作者姓名:扈廷茂  刘明秋
作者单位:内蒙古大学生物学系,内蒙古大学生物学系,内蒙古大学生物学系,内蒙古大学生物学系,内蒙古大学生物学系,内蒙古大学生物学系,内蒙古大学生物学系
摘    要:用显微注射法,将MT-hGH融合基因注入昆明白小鼠受精卵雄原核中,受注的原核卵经体外培养发育的198枚2细胞胚、45枚桑椹胚和161枚胚泡,移植到54只假孕受体,其中16只妊娠产仔只,存活到供试的仔鼠25只,小鼠长到3个月后,切尾制备DNA,经DNA斑点杂交和Southern印迹杂交检测基因整合情况,25只小鼠中3只有融合基因整合,称为转基因小鼠,仔鼠21日龄离乳后饮水中加入锌(Za^ ),以诱导融合基因表达,85日龄时,转基因小鼠体重比对照组重32.0%,还讨论了基因整合方式对胚胎发育的影响。

关 键 词:分子杂交  MT-hGH基因  受精卵

Study on the Introduction of MT-hGH Fusion Gene to Fertilized Mouse Eggs
Hu Tingmao,Liu Mingqiu,Wang Dazhen,Zhang Zhaoying,Li Xuefeng,Hu Huiping,Lu Meixia.Study on the Introduction of MT-hGH Fusion Gene to Fertilized Mouse Eggs[J].Acta Scientiarum Naturalium Universitatis Neimongol,1993,24(1):75-80.
Authors:Hu Tingmao  Liu Mingqiu  Wang Dazhen  Zhang Zhaoying  Li Xuefeng  Hu Huiping  Lu Meixia
Institution:Hu Tingmao;Liu Mingqiu;Wang Dazhen;Zhang Zhaoying;Li Xuefeng;Hu Huiping;Lu Meixia Department of Biology
Abstract:MT-hGH fusion genes were introduced to pronuclel of the fertilized eggs of mice by microin- jection. After cultivation in vitro, 198 2-cell embryos, 45 morulae and 161 blastocysts were trans- ferred into 54 pseudomothers. 16 of 54 mothers gave birth to49 litters. 25 of the litters were grown up to be three months old. The tail of each mouse was cut to isolate the DNA. DNA Was examined by dot hybridization and Southern blotting. Three mice incorporated the fusion genes and all grew larger than control mice. Synthesis of human growth hormone was further induced by zinc, which normally induces metallothionein gene expression.
Keywords:human growth hormone gene  microinjection  hybridization transgenic mice
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