| 一种血浆多肽组学样本提取方法 |
| |
| 引用本文： | 刘宇,唐东芳,彭德正,谢方,张娇飞,刘洋慧,朱政斌,易健民.一种血浆多肽组学样本提取方法[J].岳阳师范学院学报,2013(4):51-53. |
| |
| 作者姓名： | 刘宇 唐东芳 彭德正 谢方 张娇飞 刘洋慧 朱政斌 易健民 |
| |
| 作者单位： | 湖南理工学院化学化工学院,湖南岳阳414006 |
| |
| 基金项目： | 国家自然科学基金项目（21172067）;湖南省教育厅重点项目（13A032）;湖南省科技厅科技计划项目（2012SK3042）;湖南省发改委2011科技基本建设项目（湘发改高技[2011]1318号） |
| |
| 摘 要： | 采用液相色谱一质谱联用技术，对血浆多肽组学样品进行前处理和优化，建立了血浆多肽组样品收集方法．通过考察乙腈沉淀和加热变性两种方法对血浆中蛋白质去除效率，确定采用乙腈沉淀；进一步对乙腈沉淀法进行优化，发现2.5倍体积乙腈沉淀达到最佳效果，处理的样本适合血浆多肽组学分析．
|
| 关 键 词： | 血浆 多肽组 乙腈沉淀 |
Plasma Peptidome Preparation Method for Peptidomics Analysis |
| |
| LIU Yu,TANG Dong-fang,PENG De-zheng,XIE Fang,ZHANG Jiao-fei,LIU Yang-hui,ZHU Zheng-bin,YI Jian-min.Plasma Peptidome Preparation Method for Peptidomics Analysis[J].Journal of Yueyang Normal University,2013(4):51-53. |
| |
| Authors: | LIU Yu TANG Dong-fang PENG De-zheng XIE Fang ZHANG Jiao-fei LIU Yang-hui ZHU Zheng-bin YI Jian-min |
| |
| Institution: | (College of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, China) |
| |
| Abstract: | Different methods for plasma peptidome preparation in peptidomics analysis were compared in this study. Plasma proteins were either precipitated with acetonitrile or subjected to themal denaturation. The eluent was subjected to analyze by Gel filtration high pressure liquid chromatography. The results indicated that acetonitrile could effectively precipitated plasma proteins, and had the best extracting effect. 2.5 fold acetonitrile was optimal. The results showed that the method was satisfactory and would be applied in peptidomics analysis of preparation. |
| |
| Keywords: | plasma peptidome acetonitrile precipitation |
| 本文献已被 维普 等数据库收录！ |
|
