细胞自噬在植物应答盐胁迫中的作用研究

本研究以模式植物拟南芥为材料,利用生理学和遗传学手段分析了盐胁迫下细胞自噬基因和活性氧(ROS)变化的相关性.结果表明野生型拟南芥Col-0在遭受盐胁迫处理3d表现了叶片漂白的症状并且会诱导ROS的产生和积累了大量的细胞死亡.荧光定量PCR实验表明盐胁迫会诱导细胞自噬相关基因的表达,细胞自噬参与了调控植物的防御机制来响应盐胁迫.进一步的实验表明拟南芥细胞自噬突变体atg2和atg5在遭受盐胁迫处理3d表现了更加严重的叶片漂白症状并且积累大量的细胞死亡和ROS.初步表明细胞自噬主要是通过调控ROS的产生来应答盐胁迫.

Study the effect of autophagy in plants response to salt stress

Plant autophagy plays an important role in response to abiotic stress. In this study, the relationship between autophagy related genes and reactive oxygen species (ROS) production was analyzed using physiological and genetic methods in model plant Arabidopsis. The results suggest that the leaves were photo-bleached, ROS production was induced and abundant cell death was accumulated in Arabidopsis wild-type Col-0 seedlings after 3 days salt stress. Quantitative real-time PCR analysis indicated that the expression of autophagy related genes were induced during salt stress. Autophagy participate in regulation defense mechanism in response to salt stress. Further studies suggest that the leaves were seriously photo-bleached, abundant cell death and ROS were accumulated in two Arabidopsis autophagy mutant seedlings (atg 2 and atg 5) after 3 days salt stress. Taken together, our results suggest that autophagy primarily regulate the ROS production in response to salt stress.