| 拟南芥AtHHR3响应热胁迫应答的初步分析 |
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| 引用本文： | 齐辉辉,蔡潇潇,杨虹伟,王健美,刘志斌,杨 毅. 拟南芥AtHHR3响应热胁迫应答的初步分析[J]. 四川大学学报(自然科学版), 2016, 53(5): 1141-1146 |
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| 作者姓名： | 齐辉辉 蔡潇潇 杨虹伟 王健美 刘志斌 杨 毅 |
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| 作者单位： | 四川大学生命科学学院,四川大学生命科学学院,四川大学生命科学学院,四川大学生命科学学院,四川大学生命科学学院, |
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| 摘 要： | 拟南芥基因AtHHR3编码一个RING结构域的E3连接酶,通过生物信息学分析发现其可能参与植物热胁迫相关的应答.为了探索其具体的功能,构建了AtHHR3互补株系,并在DNA水平和转录水平分别鉴定了AtHHR3互补株系,用RT-PCR技术分析了AtHHR3在热处理条件下基因表达的变化情况.在热胁迫下分析了野生型、突变体athhr3、回复株系幼苗存活以及种子萌发的表型变化情况,发现突变体athhr3表现出对热胁迫的耐受性,并检测了热胁迫下不同株系的HSF、HSP等热相关基因的转录水平的变化,初步的研究表明拟南芥基因AtHHR3负调控植物对热胁迫的耐受性.
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| 关 键 词： | AtHHR3 E3连接酶 热胁迫 拟南芥 耐热性 |
| 收稿时间： | 2015-12-31 |
| 修稿时间： | 2016-04-01 |
Primary Analysis of the Function of Arabidopsis thaliana AtHHR3 to Response Heat Stress |
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| QI Hui-Hui,CAI Xiao-Xiao,YANG Hong-Wei,WANG Jian-Mei,LIU Zhi-Bin and YANG Yi. Primary Analysis of the Function of Arabidopsis thaliana AtHHR3 to Response Heat Stress[J]. Journal of Sichuan University (Natural Science Edition), 2016, 53(5): 1141-1146 |
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| Authors: | QI Hui-Hui CAI Xiao-Xiao YANG Hong-Wei WANG Jian-Mei LIU Zhi-Bin YANG Yi |
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| Affiliation: | Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University and Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University |
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| Abstract: | |
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| Keywords: | AtHHR3 E3 ligase heat stress Arabidopsis thaliana thermotolerance |
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