| 限制性酶切DNA片段的计算机辅助测量 |
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| 引用本文： | 吴晓强,钟光华. 限制性酶切DNA片段的计算机辅助测量[J]. 首都师范大学学报(自然科学版), 1991, 0(3) |
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| 作者姓名： | 吴晓强 钟光华 |
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| 作者单位： | 北京帅范学院生物系(吴晓强)，北京师范学院化学系(钟光华) |
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| 摘 要： | 文中对几种测量限制性内切酶解DNA片段大小方法的测量精度进行了比较。在所讨论的方法中有手工半对数作图法、线性回归、组合线性回归、拉格朗日插值法、Southern法及改进的分段函数法和其计算机计算程序。用以上方法检验了λDNA/HindⅢ+EcoRⅠ酶切片段的测量精度,并测量了pSaA基因限制性内切片段大小。结果表明改进的分段函数法给出最小标准方差(Sd)及最优测量一致性。
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| 关 键 词： | 限制性DNA片段 计算机模型 测量 |
Estimation of DNA Fragment Lengths by Improved Computerized Models |
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| Wu Xiaoqiang,Zhong Guanghua. Estimation of DNA Fragment Lengths by Improved Computerized Models[J]. Journal of Capital Normal University(Natural Science Edition), 1991, 0(3) |
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| Authors: | Wu Xiaoqiang Zhong Guanghua |
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| Abstract: | A comparison of the accuracy of different methods used to convert theMobility of Restr iction DNA fragments fractioned on agrose to DNA size ismade. Among the methods of Manual Plotting of Semi-Log Curve, the imp-roved model is used to achieve linear and combined linear regressions basedon the curve and related computational program, computerized programsbased on Southern Principle and Lagrange Insertion, and an improved com-puterized model based on both Semi-Log and Southern Principle for estima-tion of DNA fragment lengths fractioned on agrose gel, with best consistencyand smallest SD value. |
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| Keywords: | DNA fragment length computerized model measure |
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