A combination of sister chromatid differential staining and giemsa banding |
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| Authors: | S. Pathak A. D. Stock A. Lusby |
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| Affiliation: | (1) Department of Biology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77025 Houston, Texas, USA;(2) Present address: Graduate School of Biomedical Sciences, The University of Texas, 77025 Houston, Texas, USA |
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| Abstract: | Summary We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists. |
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