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大熊猫电刺激采精及精液冷冻保存研究
引用本文:黄炎 Howar.,J.大熊猫电刺激采精及精液冷冻保存研究[J].西华师范大学学报(哲学社会科学版),2000,21(3):238-243.
作者姓名:黄炎 Howar.  J
作者单位:[1]卧龙自然保护区大熊猫研究中心 [2]华盛顿国家动物园
摘    要:对卧龙自然保护区大熊猫研究中心的9只5.5-16.5岁的雄性大熊猫进行了17次电刺激采精,其中3只是能进行自然交配并繁殖了后代的种公兽,比较研究了精液离心、不同稀释液和冷冻方法对大熊猫精液超低温冷冻保存后的活力、运动状态、精子和顶体形态的影响。细冷冻是1种较好的超低温冷冻精液的方法。

关 键 词:大熊猫  电刺激采精  低温冷低  精液

Electroejaculation and semen cryopreservation in giant pandas
HUANG Yan ,LI De sheng ,ZHANG He min ,DU Jun ,ZHANG Gui quan ,WANG Peng yan ,Jogayle Howard ,Rrbacca Spindler ,Barbara Durrant ,Mary Ann Olson.Electroejaculation and semen cryopreservation in giant pandas[J].Journal of China West Normal University:Natural Science Edition,2000,21(3):238-243.
Authors:HUANG Yan  LI De sheng  ZHANG He min  DU Jun  ZHANG Gui quan  WANG Peng yan  Jogayle Howard  Rrbacca Spindler  Barbara Durrant  Mary Ann Olson
Institution:HUANG Yan 1,LI De sheng 1,ZHANG He min 1,DU Jun 1,ZHANG Gui quan 1,WANG Peng yan 1,Jogayle Howard 2,Rrbacca Spindler 2,Barbara Durrant 3,Mary Ann Olson 3
Abstract:Nine male pandas between 5.5 to 16.5 years old at the Research and Conservation Center for Giant Panda in Wolong Nature Reserve were used for this study. Spermatozoa was collected by electroejaculation seventeen times. Three of the males already have produced offspring through nature mating. Affects of sperm washing, cryodiluents, freezing methods (pellet, straw and vial) on sperm motility ,stahus, morpholgy and acrosome were studied. To freeze in straws was easier and faster than to freeze in pellets. It was a better method.
Keywords:giant panda  electroejuulation  freezing  semen  conserve
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