| 降解结晶纤维素极耐热葡聚糖酶重组菌的构建 |
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| 引用本文： | 李相前,邵蔚蓝. 降解结晶纤维素极耐热葡聚糖酶重组菌的构建[J]. 南京林业大学学报(自然科学版), 2006, 30(3): 37-40 |
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| 作者姓名： | 李相前 邵蔚蓝 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036;淮阴工学院生物工程与化学工程系,江苏,淮安,223001;江南大学工业生物技术教育部重点实验室,江苏,无锡,214036;南京师范大学生命科学学院,江苏,南京,210097 |
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| 摘 要： | 极耐热纤维素酶在纤维素生物转化中具有潜在的开发前景,但极耐热酶缺乏对天然纤维素的分解能力。采用基因工程方法,将极耐热内切葡聚糖酶Cel12B基因和同样极端嗜热菌来源的纤维素结合域(CBD)区域融合,构建重组质粒pET-20b-Cel12B-CBD,转化至大肠杆菌JM109(DE3)诱导表达后,融合基因表达产物对结晶纤维素具有一定的分解能力。
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| 关 键 词： | 结晶纤维素 基因融合 内切葡聚糖酶 |
| 文章编号： | 1000-2006(2006)03-0037-04 |
| 收稿时间： | 2005-11-11 |
| 修稿时间： | 2006-04-07 |
Construction of Recombinant Escherichia coli for Producing Extremely Thermostability Endoglucanase |
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| LI Xiang-qian,SHAO Wei-lan. Construction of Recombinant Escherichia coli for Producing Extremely Thermostability Endoglucanase[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2006, 30(3): 37-40 |
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| Authors: | LI Xiang-qian SHAO Wei-lan |
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| Abstract: | The endoglucanase found in Thermotoga maritima showed extremely high thermostability and considerable potential in industrial application of enzymatic hydrolysis of cellulose.Endoglucanase(Tm) Cel12B is extracellular enzyme.Tm Cel12B did not contain a cellulose-binding domain(CBD)and lacked activity on crystalline cellulose.Tm XynA is composed of catalytic domain(CD)and cellulose-binding domain(CBD).As such,the gene of CBD from Tm XynA was fused at the carboxyl-terminus of Tm Cel12B and recombinant plasmid pET-20b-Cel12B-CBD was obtained.The chimera enzyme was expressed in Escherichia coli and activities were displayed on crystalline cellulose. |
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| Keywords: | Crystalline cellulose Gene fusion Thermostability endoglucanase |
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