| 锦鲤弗氏柠檬酸杆菌的鉴定 |
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| 引用本文： | 陈翠珍,房海,葛慕湘,王秀云.锦鲤弗氏柠檬酸杆菌的鉴定[J].河北科技师范学院学报,2011,25(4):21-25,44. |
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| 作者姓名： | 陈翠珍 房海 葛慕湘 王秀云 |
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| 作者单位： | 河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛,066004 |
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| 基金项目： | 河北省科学技术研究与发展计划项目(项目编号:11960503D) |
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| 摘 要： | 对从病死锦鲤（cyprinuscarpioL．）肝组织中分离的2株菌（编号：HC050630B-1，HC050630B-2）进行了形态特征、主要理化特性、对健康鲤鱼的致病作用、药物敏感性等方面的检验；同时测定了HCOS0630B-1株菌的16SrRNA基因序列，构建了系统发育树。结果表明，2株被检菌为弗氏柠檬酸杆菌（C...
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| 关 键 词： | 锦鲤 弗氏柠檬酸杆菌 鉴定 |
Identification of Citrobacter freundii from Ornamental Carp(Cyprinus carpio L.) |
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| CHEN Cui-zhen,FANG Hai,GE Mu-xiang,WANG Xiu-yun.Identification of Citrobacter freundii from Ornamental Carp(Cyprinus carpio L.)[J].Journal of Hebei Normal University of Science & Technology,2011,25(4):21-25,44. |
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| Authors: | CHEN Cui-zhen FANG Hai GE Mu-xiang WANG Xiu-yun |
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| Institution: | (Hebei Province Key Laboratory of Veterinary Preventive Medicine,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066004,China) |
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| Abstract: | Two strains(HC050630B-1,HC050630B-2) isolated from the liver of diseased(or dead) ornamental carp were examined,including their morphological characteristics,physiological and biochemical characteristics,pathogenicity to healthy carp and antibiotic sensitivity.In addition,the 16S rRNA gene of HC050630B-1 were sequenced,and molecular phylogenetic tree was constructed.The results showed that the two examined strains were identified as Citrobacter freundii,and had strong pathogenicity to healthy carp.The length of the 16S rRNA gene sequence of examined strain is 1 458 bp.Its database accession numbers in NCBI is EF669481,and the similarity of the 16S rRNA gene sequence of examined strain with the 16S rRNA gene of Citrobacter from GenBank database is 99%.The antibiotic sensitivity using 37 antimicrobial agents showed that isolates were high sensitive or sensitive to 21 agents including cefotaxime,slight sensitive to 5 agents including cefazolin,and resistant to 11 agents including tetrecycline. |
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| Keywords: | Cyprinus carpio L Citrobacter freundii identification |
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