Anti-myosin stains chromaffin cells

Summary The presence in fixed chromaffin cells of antigenic sites for a myosin antibody was demonstrated using immunofluorescence techniques. Tests on viable cells showed that at least some of the antigenic sites seem to be localized on or close to the cell surface and explained the cell agglutination that occurred with the addition of the myosin antibody to cells isolated by a method described in this paper.Acknowledgments. This work was supported by grants from the Medical Research Council of Canada. We thank Dr B. G. Livett for advice in the preparation of isolated cells, Drs J. Lowenthal and B. Collier for comments and Mrs J. Ritchie for technical assistance.