增强草鱼免疫功能的复方中草药免疫增强剂筛选

选取920尾草鱼,平均体质量为(62.15±9.78)g,随机分成4个组,每组230尾鱼,其中1个对照组(投喂基础饲料),3个试验组(投喂含中草药免疫增强剂的饲料:基础饲料中分别添加20g/kg中草药免疫增强剂1(T1),2(T2)和3(T3)).每组分别在第0,7,14,21和28d采样测定免疫指标,饲养30d后进行人工感染试验.结果表明各试验组较对照组在吞噬细胞吞噬活力、血清溶菌活力、血清抗菌活力、血清超氧化物岐化酶(SOD)活力和血清过氧化物酶(POD)活力方面均显著升高(p<0.05).T1组、T2组和T3组抗多子小瓜虫感染的免疫保护率分别为0,100%±0.00%和42.50%±6.61%.T1组、T2组和T3组对注射嗜水气单胞菌的免疫保护率分别为15.37%±8.36%,78.43%±8.77%和63.06%±3.37%.研究表明在基础饲料中添加2%复方2可显著提高草鱼的免疫功能和抗病力(p<0.05),在3种试验复方中效果最佳,在生产中有应用价值.

Identification of Compound Chinese Herbal Immunostimulants Enhancing the Immunity of Grass Carp (Ctenopharyngodon Idellus)

Nine hundred and twenty healthy grass carp at the average weight of 62.15±9.78 g are randomly chosen and divided into four groups(230 carp in each group).Of the four groups of grass carp,one group serves as contrast group(which are fed with the basic fodder) and the other 3 trial groups(groups T1,T2 and T3),which are fed with basal diets supplemented with 20 g/kg of the compound immunostimulant 1,2 and 3,respectively.The carp in control group are fed with just basal diet(without compound immunostimulants).Each group is randomly sampled at 0,7,14,21 and 28 days to detect the periphery blood immune parameters.Challenge experiment is carried out on the thirtieth day.The results are as follows: The activity in phagocyte phagocytic,serum lysozyme,serum antibacterial,SOD,and POD in serum is all significantly(p<0.05) enhanced in the group T1,group T2,and group T3 when compared with the control group.When grass carp are injected with live Aeromonas hydrophila,the relative percentage survival(RPS) of the fish was 15.37%±8.36%,78.43%±8.77%,and 63.06%±3.37% for group T1,T2 and T3,respectively.While grass carp was challenged with theronts of Ichthyophthirius multifiliis,the relative percentage survival(RPS) of the fish is 0,100%±0.00%,and 42.50%±6.61% for groups T1,T2,and T3,respectively.This research indicates that basal diet which is added 2% of Chinese herbal immunostimulant 2 can significantly improve the immune function and disease resistance of grass carp(p<0.05).It can be concluded that Chinese herbal immunostimulant 2 is the most effective immunostimulant in the 3 test compound products.