电导法结合Logistic方程鉴定三倍体枇杷的抗寒性研究

以三倍体枇杷成熟叶片为试材,经低温处理后采用电导法测定质膜相对电导率,然后利用Logistic方程拟合拐点得到单株半致死温度,结果表明:不同低温处理后,叶片组织的相对电导率与处理温度呈负相关,整体变化为典型S形单峰曲线;在-5℃到-15℃之间,质膜伤害率随温度下降而急剧升高;单株半致死温度在-1.22℃到-10.47℃之间.单株抗寒力"常白1号Q34"最强,"东湖早"最弱.

Measurement of Cold Tolerance Based on REC and the Logistic Equation in Triploid Loquat

In order to investigate the semi-lethal temperature(LT50) for triploid loquat,the changes of cell membrane permeability at different low temperatures were studied with triploid loquat mature leaves.The relative electric conductivity(REC) appeared in a significant negative correlation with the treatment temperature and its variations might be described with a typical S-shaped curve with a single peak.Within the range of -5 ℃ to -15 ℃,leakage ratio of electrolyte increased sharply with decreasing temperature.The semi-lethal temperature for the tested materials ranged from -1.22 ℃ to -10.47 ℃.Of the loquat varieties studied,ChangbaiQ34 showed the highest cold tolerance and Donghuzao had the weakest.