Acetylcholinesterase activity in anAedes aegypti cell line |
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Authors: | E Cohen |
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Institution: | (1) Department of Entomology, The Hebrew University of Jerusalem, Faculty of Agriculture, P.O. Box 12, Rehovot, (Israel) |
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Abstract: | Summary Considerable acetylcholinesterase (AChE) activity was detected in anAedes aegypti established cell line. The enzyme is blocked by 10–6 M eserine sulfate, displays excess substrate inhibition and slowly hydrolyzes butyrylthiocholine. A 2-fold stimulation of AChE activity was shown after 2 days exposure to 3×10–7 M -ecdysone. AChE activity found in the fresh medium is the contribution of the fetal calf serum portion. A direct relationship between levels of serum and the AChE activity in the cultured cells was demonstrated.Acknowledgment. I wish to thank Dr J. Peleg of the Israel Institute for Biological Research for providing the starting culture ofAedes aegypti cells. |
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