矮坨坨内生真菌Fusarium oxysporum代谢产物及其抗肝癌活性的研究

从矮坨坨根部分离得到的一株内生菌，经形态与分子生物学18S rDNA序列分析鉴定为尖孢镰刀菌(Fusarium oxysporum).运用中压柱色谱，高效制备液相色谱方法和现代波谱技术1HNMR、13CNMR以及LC-MS从其发酵液的乙酸乙酯部位分离鉴定了9个单体化合物，分别为4-氨基-2-羟基嘧啶(1),4,5-二氢-3H-吡唑-3-羧酸甲酯(2),4-羟基-7-(2-羟基-丁基)-3-甲基-氧杂环庚烷-2-酮(3),2-氨基-3-甲基戊酸(4),2,5-二氨基戊酸(5),2-氨基-3-甲基丁酸(6)4-羟基苯甲酸(7)，脱氢镰刀菌酸(8)，镰刀菌酸(9)，其中化合物8对肝癌HepG2和Hep3B细胞具有较强的细胞毒活性，IC50值分别为23.9和29.1 μg/mL，而对正常肝细胞L-02未显示细胞毒性.

Study on the metabolites and their anti-hepatoma activity of Fusarium oxysporum isolated from Munronia delavayi Franch.

In this study， the endophytic fungus isolated from the roots of Munronia delavayi Franch. was determined as Fusarium oxysporum based on fungus's morphology and 18S rDNA sequence analysis. The isolation and purification of the ethyl acetate extract of fermentation was performed with medium-pressure column chromatography and preparative HPLC. The structures of the nine compounds were identified by extensive spectroscopic analysis as 1HNMR， 13CNMR and LC-MS. Their structures were elucidated as 4-amino-2-hydroxypyrimidinel (1)， 4,5-Dihydro-3H-pyrazole-3-carboxylic acid methyl (2)， 4-hydroxy-7-(2-hydroxy-butyl)-3-methyl-oxepan-2-ketone (3)， 2-amino-3-methyl-pentanoic acid (4)， 2,5-diamino-pentanoic acid (5)， 2-amino-3-methyl-butyric acid (6)， 4-hydroxy-benzoic acid (7)， dehydrofusaric acid (8) and fusaric acid (9). Compound 8 exhibited cytotoxic activity against HepG2 and Hep3B cell lines with IC50 values of 23.9 and 29.1 μg/mL， respectively. However， the cytotoxicity was not shown in L-02 of normal liver cells.