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| PCR快速检测常见具有毒性质粒的沙门氏菌 | |
| 引用本文： | 马立农,刘华伟,陈跃进,曾智. PCR快速检测常见具有毒性质粒的沙门氏菌[J]. 世界科技研究与发展, 2011, 33(1): 36-38 |
| 作者姓名： | 马立农 刘华伟 陈跃进 曾智 |
| 作者单位： | 1. 深圳职业技术学院,深圳,518055 2. 西北农林科技大学,杨凌,712100 3. 湖南人文科技学院,娄底,417000 |
| 摘 要： | 针对常见具毒性质粒的沙门氏菌的spvR基因，设计出PCR特异引物spvRP35-P36，以便快速检测出具有毒性质粒的沙门氏菌。验证采用13株沙门氏菌和4株非沙门氏菌，其中6株常见具有毒性质粒的沙门氏菌均获得特异性扩增，7株不舍毒性质粒的沙门氏菌均未获得特异性扩增，4株非沙门氏茵检测结果为阴性。结果表明，本文中设计的引物具有高度的特异性，适用于常见具有毒性质粒沙门氏菌的快速检测，此引物已获国家发明专利。 |
| 关 键 词： | 沙门氏菌 毒性质粒 基因扩增 特异引物 |
Rapid Detection of Salmonella Virulence-Associated Plasmid Genes by PCR |
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| MA Linong,LIU Huawei,CHEN Yuejin,ZENG Zhi. Rapid Detection of Salmonella Virulence-Associated Plasmid Genes by PCR[J]. World Sci-tech R & D, 2011, 33(1): 36-38 | |
| Authors: | MA Linong LIU Huawei CHEN Yuejin ZENG Zhi |
| Affiliation: | 1. Shenzhen Polytechnic, Shenzhen 518055 ;2. Northwest of Agriculture and Forestry University,Yangling 712100; 3. Hunan Institute of Humanities, Science and Technology, Loudi 417000) |
| Abstract: | For detecting of virulence-associated plasmid genes is very important in assessing virulence for Salmonella, a pair of specific oligonucleotide primers is designed. And a detection method is developed by PCR. Thirteen strains of Salmonella and four strains of other bacterial species are tested by PCR using a pair of oligonucleotide primers spvR-P35-P36. Six toxic Salmonella strains containing spvR genes in virulenee-asseciated plasmid are positively identified. Seven non-toxic Salmonella strains and four non-Salmonella strains are all negative as determined by the PCR method,which well suits for the detection of toxic Salmonella strains based on the existence of virulence-associated plasmid genes. It is awarded the country invention patent. |
| Keywords: | Salmonella virulence-associated plasmid gene amplification specific oligonucleotide primers |
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