Cytoskeletal filaments of heart conducting system localized by antibody against a 55,000 dalton protein |
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| Authors: | A Eriksson L -E Thornell T Stigbrand |
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| Institution: | (1) Department of Anatomy, University of Umeå, S-90187 Umeå, (Sweden);(2) Department of Physiological Chemistry, University of Umeå, S-90187 Umeå, (Sweden) |
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| Abstract: | Summary Cow heart conducting cells characteristically contain cytoplasmic intermediate-sized filaments. We report here the preparation of a specific antibody to a 55,000 dalton protein of isolated cow Purkinje fibres. Confirmation has been obtained that these filaments consist of the 55,000 dalton protein, using the indirect immunofluorescence technique. Crossreaction is seen with vascular endothelium and smooth muscle cells of various origin, suggesting close identity of different types of intermediate-sized filaments. |
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