木兰叶绿体atpB和rbcL基因的系统进化分析

木兰科植物作为古老的类群，其杂交和形态多样以及趋同进化等特点导致传统分类出现很多不同结论。对生物类群进行种群的系统进化分析可以在一定程度上弥补传统分类方法的局限。叶绿体的母系遗传相对保守、分子水平差异明显，是绿色植物的标志性细胞器，因此叶绿体基因组常被用来进行植物的系统进化探究。本项研究利用phylip软件将NCBI核酸序列数据库中下载的21种木兰科植物叶绿体的atpB和rbcL基因序列760bp进行序列比对处理之后，进一步应用邻接法进行分子进化系统发生分析，构建系统发育进化树，从而得出木兰属中白玉兰、星花玉兰、锐叶木兰、北美大叶木兰、夜香木兰等在系统进化中的亲缘关系，为进一步对木兰属植物进行系统发生研究奠定基础。

Evolution Analysis of the Chloroplast Genes atpB and rbcL of Magnolia

Magnoliaceae plants are ancient plant groups. Due to the hybridization, diversity of morphology, and convergent evolution, many different conclusions of the classical classification were emerged. Phylogenetic analysis of species on biological groups could com-pensate for the limitations of classical classification. The matrilineal genetic of chloroplast is relatively conservative, and it has significant differences at molecular level. Chloroplast is iconic green plant organelles, and the chloroplast genome is commonly used in phylogenetic analysis. In this study, phylip software was used for the sequence alignment on 760bp of genes atpB and rbcL of 21species of the Magno-lia chloroplast, which were downloaded from the International Nucleotide Sequence Databank. The neighbor-Joining method was used for molecular and phylogenetic analysis and reconstruction of a phylogenetic tree,and the genetic relationships in phyletic evolution among Magnolia denudate, Magnolia stellata, Magnolia acumirata, Section macrophylla, and Magnolia coco were gotted. It could lay the foun-dation for phylogenetic study on Magnolia.