| 乙醇系统反相高效液相色谱分析DABS－氨基酸 |
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| 引用本文： | 陈平,梁宋平.乙醇系统反相高效液相色谱分析DABS－氨基酸[J].湖南师范大学自然科学学报,1996(3). |
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| 作者姓名： | 陈平 梁宋平 |
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| 作者单位： | 湖南省生物研究所 |
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| 摘 要： | 采用对二甲氨基偶氮苯磺酰氯（ＤＡＢＳ－Ｃｌ）衍生法分析氨基酸组成．利用醋酸盐／乙醇系统对衍生的氨基酸进行ＨＰＬＣ分离．用乙醇取代乙腈作为层析剂可减少毒性，降低成本．与常用的异硫氰酸苯酯（ＰＩＴＣ）衍生法相比，实验证明ＤＡＢＳ－Ｃｌ衍生法具有高灵敏度、高稳定性和操作简便的优点．
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| 关 键 词： | 氨基酸分析 DABS－氨基酸 反相高效液相色谱 |
The Analysis of DABS-amino Acids by High-Performance Liquid Chromatography Using An Alcohol Elution System |
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| Chen Ping Liang Sorlgping.The Analysis of DABS-amino Acids by High-Performance Liquid Chromatography Using An Alcohol Elution System[J].Journal of Natural Science of Hunan Normal University,1996(3). |
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| Authors: | Chen Ping Liang Sorlgping |
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| Abstract: | A method of amino acid analysis through derivatization with dimethylaminoazozene sulphonyl chloride(DABS-Cl)is reported.The DABS-amino acids were analysed by HPLC using an acetate/alcohol elution system,which is economical and less toxicthan the commonly used acetate/acetonitrile system.The result indicate that this method hasthe advantage of high sensitivity and stability than the method of derivatization of amino acidby PITC. |
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| Keywords: | amino acid analysis DABS-amino acid reversed-phase High-performanceLiquid Chromatographic |
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