| 成都动物园野生动物源贾第虫的多位点基因分型鉴定 |
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| 引用本文： | 李威,屈羽,钟志军,杨平,李云娇,王吴优,刘学涵,谢娜,邓家波,彭广能.成都动物园野生动物源贾第虫的多位点基因分型鉴定[J].四川大学学报(自然科学版),2017,54(1):185-190. |
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| 作者姓名： | 李威 屈羽 钟志军 杨平 李云娇 王吴优 刘学涵 谢娜 邓家波 彭广能 |
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| 作者单位： | 四川农业大学,,,,,,,, |
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| 基金项目： | 国家自然科学基金,其它 |
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| 摘 要： | 为了解四川省成都市动物园野生动物贾第虫的流行及基因型,本研究采集了146份不同野生动物的新鲜粪便并提取基因组DNA.通过巢式PCR扩增β-giardin、tpi和gdh基因,扩增产物测序后进行种系发育分析.结果表明,CDZOO1黇鹿源和CDZOO3龟源贾第虫通过多位点基因分型(MLG)鉴定为AI-1亚型;CDZOO2鹿源贾第虫为E型(β-giardin基因位点);CDZOO4黇鹿源贾第虫为A型(β-giardin和tpi基因位点);CDZOO5浣熊源和CDZOO6细尾獴源贾第虫在β-giardin位点为D型而在tpi位点为A型.
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| 关 键 词： | 贾第虫 β-giardin基因 tpi基因 gdh基因 MLG 种系发育 人畜共患 |
| 收稿时间： | 2015/5/26 0:00:00 |
| 修稿时间： | 2016/3/17 0:00:00 |
Multilocus genotype (MLG) analysis of Giardia from captive wildlife in Chendu zoo |
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| Institution: | Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Chengdu Zoo, Institute of Wild Animals in Sichuan Province,Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Mingshan Agricultural Department of Ya''an City in Sichuan Province,Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University,Chengdu Zoo, Institute of Wild Animals in Sichuan Province and Key Laboratory of Animal Disease and Human Health of Sichuan Province /College of Veterinary Medicine, Sichuan Agricultural University |
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