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| 暗纹东方鲀线粒体若干tRNA基因的克隆及序列分析 | |
| 引用本文： | 邵爱华,叶亚新,王金虎,朱江. 暗纹东方鲀线粒体若干tRNA基因的克隆及序列分析[J]. 苏州科技学院学报(自然科学版), 2006, 23(4): 37-42 |
| 作者姓名： | 邵爱华 叶亚新 王金虎 朱江 |
| 作者单位： | 1. 苏州科技学院,生物系,江苏,苏州,215009;苏州大学,生命科学学院,江苏,苏州,215123 2. 苏州科技学院,生物系,江苏,苏州,215009 3. 苏州大学,生命科学学院,江苏,苏州,215123 |
| 基金项目： | 农业部行业标准制定和修订项目;苏州科技学院校科研和教改项目 |
| 摘 要： | 利用分离纯化的暗纹东方鲀肝脏mtDNA为模板，按照红鳍东方纯（Takifugu rubripes）mtDNA序列设计合成特异引物进行PCR扩增，首次克隆并测定了暗纹东方纯mtDNA的Cytb、COⅠ、COⅡ、COⅢ、D-loop等5个重要基因及其侧翼的8个tRNA基因。上述基因均已在GenBank登录。8个tRNA基因含有69-72个碱基。推定了各个tRNA的二级结构并进行了初步的序列分析。结果表明：8个tRNA基因具有较为典型的三叶草型结构，各臂的碱基配对率大多数较高，稳定性较好. |
| 关 键 词： | 暗纹东方鲀 线粒体tRNA基因 二级结构 |
| 文章编号： | 1672-0679(2006)04-0037-06 |
| 收稿时间： | 2006-06-06 |
| 修稿时间： | 2006-06-06 |
Cloning and Sequence Analysis of the tRNA Genes of Mitochondrial Genome from Takifugu fasciatus |
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| SHAO Ai-hua,YE Ya-xin,WANG Jin-hu,ZHU Jiang. Cloning and Sequence Analysis of the tRNA Genes of Mitochondrial Genome from Takifugu fasciatus[J]. Journal of University of Science and Technology of Suzhou, 2006, 23(4): 37-42 | |
| Authors: | SHAO Ai-hua YE Ya-xin WANG Jin-hu ZHU Jiang |
| Affiliation: | 1. Dept. of Biology, USTS, Suzhou 215009, China; 2. Life Science School, Soochow University, Suzhou 215123, China |
| Abstract: | Mitochondrial DNA of Takifugu fasciatus is prepared as templates from the liver tissue.Several important genes,such as cytochrome b(Cytb) and cytochrome oxidase subunit I,II,III(COI,COII,COIII) and D-loop,and their connected tRNA genes are amplified,cloned and measured by PCR separately with a pair of specific primers which designed according to the sequence of Takifugu rubripes are released from Genbank.All of them have been registered in GenBank.The eight tRNA genes contain nucleotides from 69 to 72.The secondary structures of tRNA genes have been deduced respectively and their sequences have been primarily analyzed.It is suggested that most of them have classical cloverleaf structures,each arm with high base pair ratio and good stability. |
| Keywords: | Takifugu fasciatus mtDNA tRNA genes secondary structure |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |