利用重组自交系群体进行抗玉米矮花叶病研究

玉米矮花叶病在世界范围内广泛分布，且危害十分严重．因此正确认识玉米（Zea mays L．）对矮花叶病的抗性机制非常必要．基于此，本研究利用遗传差异较大的Mo17（高感玉米矮花叶病）和黄早四（高抗玉米矮花叶病）为亲本采用一粒传法构建了F9代重组自交系（Recombinant inbred line，RIL）分离群体，共256系．通过人工接甘蔗花叶病毒MDB株系（Sugarcane mosaic virus strain MDB, SCMV-MDB）对该分离群体进行了抗病性分析，统计了发病率和病情指数两项指标，根据这两项指标的频数分布图可知：玉米对矮花叶病的抗性主要由主基因控制，但不能排除还有微效基因的可能性．

Research on Resistance to Maize Dwarf Mosaic Disease Using Recombinant Inbred Line in Maize

The maize dwarf mosaic disease(MDMD),distributing widely in the world,causes serious yield loss in maize.Thus,it is very necessary to understand the mechanism of resistance to MDMD.In the study,A F_9 recombinant inbred line(RIL) population including 256 RILs derived from a cross of Mo17(highly susceptible) and Huangzao4(highly resistant),was constructed with the method of one-kernel heredity.The 256 RILs and their parental lines were evaluated for their resistance to SCMV-MDB under artificial inoculation.According to the frequency distribution graphs of percentage of disease symptom and disease index,the resistance to SCMV-MDB for maize was controlled by major genes,but could not exclude the possibility of other minor genes.