| Isolation and mutation rate of the spontaneous PUR box and purR mutants |
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| 作者姓名： | DAI Shipan YANG Zhiwei QIN Junchuan WANG Aoquan |
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| 作者单位： | National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China,National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant No.39970008) |
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| 摘 要： | In our previous research in purine metabolism in Salmonella typhimurium, it was observed that the mutation frequency of the PUR box was one order of magnitude higher than that of purR under mutagenesis. In order to investigate further into this phenomenon, large amounts of independent PUR box and purR spontaneous mutants were isolated on lactose minimal medium by using a super-repressing mutant of purR (purRs). The mutational regions of 5 PUR box mutants and 4 purR mutants were cloned. Nucleotide sequence analysis showed that all the mutants had mutations at the expected sites. The comparison of the two types of mutations indicated that, although the purR gene was two orders of magnitude larger than the box). Thus, we concluded that high mutation frequency of the PUR box did not result from mutagenesis. Under spontaneous conditions, the mutation frequency of PUR box was also high. Some tentative explanations of this interesting phenomenon are given in this report.
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| 关 键 词： | super-repressing mutant PUR box purR |
Isolation and mutation rate of the spontaneous PUR box and purR mutants |
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| DAI Shipan,YANG Zhiwei,QIN Junchuan,WANG Aoquan.Isolation and mutation rate of the spontaneous PUR box and purR mutants[J].Progress in Natural Science,2002,12(5):359-363. |
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| Authors: | DAI Shipan YANG Zhiwei QIN Junchuan WANG Aoquan |
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| Institution: | 1. National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | In our previous research in purine metabolism in Salmonella typhimurium, it was observed that the mutation frequency of the PUR box was one order of magnitude higher than that of purR under mutagenesis. In order to investigate further into this phenomenon, large amounts of independent PUR box and purR spontaneous mutants were isolated on lactose minimal medium by using a super-repressing mutant of purR (purRs). The mutational regions of 5 PUR box mutants and 4 purR mutants were cloned. Nucleotide sequence analysis showed that all the mutants had mutations at the expected sites. The comparison of the two types of mutations indicated that, although the purR gene was two orders of magnitude larger than the box). Thus, we concluded that high mutation frequency of the PUR box did not result from mutagenesis. Under spontaneous conditions, the mutation frequency of PUR box was also high. Some tentative explanations of this interesting phenomenon are given in this report. |
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| Keywords: | super-repressing mutant PUR box purR |
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