Epithalon对人胎肝细胞端粒酶活性和端粒长度的影响

以松果体分泌物,"Epithalamin"缩氨酸为基础,人工合成多肽"Epithalon"。通过用细胞形态学观察用药后细胞的生长情况,MTT法检测Epithalon多肽对人肝细胞株L-02增殖与活力的影响,以及运用端粒酶重复序列扩增——焦磷酸根酶联发光技术检测人肝细胞株L-02端粒酶活性,流式荧光原位杂交法检测端粒长度。研究了多肽作用于人肝细胞L-02后对细胞的生长情况、细胞端粒长度以及细胞端粒酶活性所产生的影响。研究显示Epithalon多肽具有提高端粒酶活性,延缓端粒缩短的作用。

Effect of Epithalon on celluar telomere and tlomerase

The synthetic polypeptide Epithalon was synthesized based on an acid compound of Epithalamin which is the secretion of conarium.Cellular morphology observation and the Thiazolyl blue(MTT) method were used to investigate the effects of Epithalon polypeptide on the growth and multiplication of L-20 cells.An enzymatic luminometric inorganic pyrophosphate detection assay(TRAP-ELIDA) was used to measure the telomerase activity of L-02 cells.Flow cytometry and fluorescence in situ hybridization(FLOW-FISH) was used to measure the telomere length.The research shows that the Epithalon polypeptide can activate cellular telomerase and slow down the shortening of the telomere length.