响应面法优化地榆根多酚提取工艺

以吉林地区地榆根为原料,采用响应面法优化超声波辅助提取地榆根多酚的工艺条件.在原料粒度、超声功率、超声时间、液料比、乙醇体积分数单因素试验的基础上,进行Box-Behnken设计,优选出超声波提取地榆根多酚的最佳工艺条件:原料粒度380~250μm、超声功率324.8 W、超声时间19.65 min、液料比[V(液)∶m(料)]15.75∶1、乙醇体积分数51.5%,多酚提取率为6.546%,与预测值吻合,由此表明此响应面模型对地榆根多酚提取具有良好的预测作用.

Extracting Polyphenols from Sanguisorba officinalis Root by Response Surface Methodology

Using Sanguisorba officinalis root as raw material in Jilin area, the polyphenols of Sanguisorba officinalis root were extracted with optimization of ultrasonic-assisted,and the response surface method was used to optimize the extraction technology parameters based on single factor experiment of raw material granularity, ultrasonic power, ultrasonic time, solvent-to-solid, ethanol concentration, etc, Box-Behnken design was done to study the optimal extraction conditions of polyphenols:the raw material granularity of 380 ~ 250 μm, the ultrasonic power of 324. 8 W,the ultrasonic time of 19. 65 min,the solvent-to-solid of 15. 75 ∶ 1[V(solvent) ∶ m (material)],the volume fraction of ethanol 51. 5%,the extraction rate of polyphenols was 6. 546%. The result was consistent with the simulated predictive value, and this suggests that the response surface model has good predicted effect on the polyphenol extraction from Sanguisorba officinalis root.