低剂量电离辐射对Ⅰ型糖尿病小鼠血糖和肾功能影响的实验研究

目的采用25 m Gy低剂量X射线照射Ⅰ型糖尿病小鼠,检测不同时间点小鼠体质量、血糖和肾功能改变情况,为低剂量照射用于预防和治疗糖尿病提供实验证据.方法建立Ⅰ型糖尿病小鼠动物模型,用血糖仪和全自动生化监测仪测量血糖、检测尿液中尿肌酐及尿白蛋白的含量.结果Ⅰ型糖尿病小鼠体质量显著低于非糖尿病组(P0.05).给予剂量为25 m Gy X射线照射后,各组小鼠体质量无显著性差异.低剂量照射使糖尿病组小鼠血糖水平受到了抑制,显著低于糖尿病组(P0.05).照射后2~16周,糖尿病组尿肌酐、白蛋白含量与糖尿病照射组比较差异具有统计学意义(P0.05).结论低剂量照射对Ⅰ型糖尿病小鼠具有保护作用,能显著降低小鼠血糖水平,还能有效降低Ⅰ型糖尿病小鼠尿中白蛋白含量,促进尿肌酐的排出,从而缓解由糖尿病高血糖引起的肾脏损伤.

Experimental Study on the Influence of Low Doses Ionizing Radiation on Blood Glucose and Renal Function in Diabetes Mice

Objective Provide experimental evidences for the effect of low dose irradiation on the prevention and treatment of diabetes,by examining body weight,blood glucose level and renal function changes in typeⅠmice at different times. Method After type Ⅰ diabetes mice model was established, blood glucose level, contents of creatinine and albumin in urine of the mice were measured with glucose meter and automatic biochemical monitor. Results The weight of typeⅠdiabetes mice were significantly lower than that of the non-diabetic group(P<0. 05). Under 25 mGy X-ray,the body weight of each group had no significant difference in mice. Low dose irradiation restrained the blood glucose level in diabetic mice,which was significantly lower than that of the model group(P<0. 05). From week 2 to week 16 after radiation,the concentrations of urine creatinine and urinary albumin showed statistical differences in the diabetes mice comparing with the X-ray exposure groups (P<0. 05). Conclusion Low dose irradiation was able to protect the typeⅠdiabetes mice,their blood glucose level decreased obviously,which can promote the elimination of urine creatinine and urinary albumin,and relieve their kidney damage caused by high blood glucose level.