| 大岩桐叶柄诱导再生植株 |
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| 引用本文： | 王家远,罗荣芬,周大刚,田蕾,张宇斌,龚宁.大岩桐叶柄诱导再生植株[J].贵州师范大学学报(自然科学版),2004,22(4):19-21. |
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| 作者姓名： | 王家远 罗荣芬 周大刚 田蕾 张宇斌 龚宁 |
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| 作者单位： | 贵州师范大学,生物技术与工程学院,贵州,贵阳,550001 |
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| 基金项目： | 国家教育部专项基金(教计司[2000]65号)，贵州师范大学学生科研基金支助 |
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| 摘 要： | 用大岩桐叶柄作为外植体,分别对试管苗诱导、增殖和生根条件进行了初步研究。结果表明:适宜诱导培养基以MS+6-BA2 0mg·L-1+NAA0 2mg·L-1+3%蔗糖+0 7%琼脂最佳,35d其诱导率为1 324,增殖培养基以MS+6-BA2 0mg·L-1+NAA0 1mg·L-1+3%蔗糖+0 7%琼脂为最好,30d其增殖倍数可达5 3倍。生根培养基以1/2MS+NAA0 6mg·L-1+3%蔗糖+0 7%琼脂为最好,生根率可达89 7%。
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| 关 键 词： | 大岩桐 叶柄 再生植株 NAA 6-BA |
| 文章编号： | 1004-5570(2004)04-0019-03 |
| 修稿时间： | 2004年6月7日 |
Abduction and plantet regeneration of leafstalk of Sinningia speciosa |
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| WANG Jia-yuan,LUO Rong-fen,ZHOU Da-gang,TIAN Lei,ZHANG Yu-bin,GONG Ning.Abduction and plantet regeneration of leafstalk of Sinningia speciosa[J].Journal of Guizhou Normal University(Natural Sciences),2004,22(4):19-21. |
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| Authors: | WANG Jia-yuan LUO Rong-fen ZHOU Da-gang TIAN Lei ZHANG Yu-bin GONG Ning |
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| Abstract: | The explant was leafstalk of Sinningia speciosa.The conditions of abduction,multiplication and rooting were studied.The results show that the optimum medium for abduction is MS with 2.0mg·L~(-1)6-BA,0.2mg·L~(-1)NAA,3% sucrose and 0.7% agar,which makes the abduction rate reach 1.324 times during 35 days, The optimum medium for shoot buds multiplication is MS with 2.0mg·L~(-1)6-BA,0.1mg·L~(-1)NAA,3% sucrose and 0.7% agar,which makes the multiplication rate reach 5.3 times during 30 days.The optimum medium for rooting is 1/2Ms with 0.6mg·L~(-1)NAA,3% sucrose,0.7% agar,which makes the rooting rate reach 89.7%. |
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| Keywords: | Sinningia speciosa leafstalk plantet regeneration NAA 6-BA |
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